PMID: 8955306Dec 1, 1996Paper

Analysis of the CO dehydrogenase/acetyl-coenzyme A synthase operon of Methanosarcina thermophila

Journal of Bacteriology
J A Maupin-Furlow, J G Ferry

Abstract

The cdhABC genes encoding the respective alpha, epsilon, and beta subunits of the five-subunit (alpha, beta, gamma, delta, and epsilon) CO dehydrogenase/acetyl-coenzyme synthase (CODH/ACS) complex from Methanosarcina thermophila were cloned and sequenced. Northern (RNA) blot analyses indicated that the cdh genes encoding the five subunits and an open reading frame (ORF1) with unknown function are cotranscribed during growth on acetate. Northern blot and primer extension analyses suggested that mRNA processing and multiple promoters may be involved in cdh transcript synthesis. The putative CdhA (alpha subunit) and CdhB (epsilon subunit) proteins each have 40% identity to CdhA and CdhB of the CODH/ACS complex from Methanosaeta soehngenii. The cdhC gene encodes the beta subunit (CdhC) of the CODH/ACS complex from M. thermophila. The N-terminal 397 amino acids of CdhC are 42% identical to the C-terminal half of the alpha subunit of CODH/ACS from the acetogenic anaerobe Clostridium thermoaceticum. Sequence analysis suggested potential structures and functions for the previously uncharacterized beta subunit from M. thermophila. The deduced protein sequence of ORF1, located between the cdhC and cdhD genes, has 29% identity to NifH2 fr...Continue Reading

References

Oct 1, 1968·The Biochemical Journal·R Pitt-Rivers, F S Impiombato
Dec 1, 1995·The Journal of Biological Chemistry·J A Maupin-Furlow, J G Ferry
Jan 1, 1995·Annual Review of Microbiology·J G Ferry
Apr 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·D R Abbanat, J G Ferry

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Citations

Mar 17, 1999·FEMS Microbiology Reviews·J G Ferry
Dec 20, 2005·Molecular Microbiology·Anirban MahapatraJoseph A Krzycki
Jun 12, 2013·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Filipa L SousaWilliam F Martin
Oct 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·H K LokeP A Lindahl
Nov 15, 2006·Proceedings of the National Academy of Sciences of the United States of America·Daniel J LessnerJames G Ferry
Mar 15, 2019·The ISME Journal·Amy R SmithFrederick S Colwell
Jul 18, 2008·Environmental Microbiology·Elizabeth PierceStephen W Ragsdale
Oct 7, 2003·Annual Review of Biochemistry·Ruma Banerjee, Stephen W Ragsdale
Jun 8, 2011·Metallomics : Integrated Biometal Science·Güneş BenderStephen W Ragsdale
Jun 27, 1998·Journal of Bacteriology·S A BurkeJ A Krzycki
Mar 27, 1998·Journal of Bacteriology·P S KesslerJ A Leigh
Jun 28, 2003·FEMS Microbiology Reviews·Scott B Mulrooney, Robert P Hausinger

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