Analysis of the cytotoxic effects of combined ultrasound, microbubble and nucleoside analog combinations on pancreatic cells in vitro

Ultrasonics
Julia MarigliaRaffi Karshafian

Abstract

Ultrasonically-stimulated microbubbles enhance the therapeutic effects of various chemotherapy drugs. However, the application of ultrasound and microbubbles (USMB) for enhancing the therapeutic effect of nucleoside analogs, which are used as front-line treatments in a range of cancers, and its underlying mechanism is not well understood. This study investigated the effect of gemcitabine, a nucleoside analog drug, in combination with USMB in increasing cell cytotoxicity relative to either treatment alone in BxPC3 pancreatic cancer cells. Cells were sonicated using low frequency (0.5 MHz) ultrasound in combination with Definity® microbubbles (1.7% v/v) in the presence of 1 µM of gemcitabine for a total of 2 h. USMB in combination with gemcitabine decreased cell viability (48 h) to 44.7 ± 5.2%, 27.7 ± 4.3%, and 12.5 ± 3.4% with increasing ultrasound peak negative pressures (220, 360, 530 kPa) from 84.7 ± 3.6%, 54.2 ± 3.8%, and 26.8 ± 3.0%, respectively, when USMB was applied in the absence of drug. We further confirmed that USMB did not enhance the internalization of 1 µM of a radiolabeled nucleoside analog (2-chloroadenosine) at each of the three chosen ultrasound PNPs, determined by radiolabeled scintillation counting. These da...Continue Reading

Citations

Apr 2, 2019·Journal of Zhejiang University. Science. B·Li-Ying Wang, Shu-Sen Zheng
Dec 25, 2019·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Tormod K BjånesBettina Riedel
Feb 13, 2020·Pharmaceutics·Tormod BjånesEmmet McCormack
Jul 10, 2019·Frontiers in Cell and Developmental Biology·Subothan Inpanathan, Roberto J Botelho
Jul 27, 2021·Ultrasound in Medicine & Biology·Lixia Mei, Zhen Zhang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Imaging

Imaging techniques, including CT and MR, have become essential to tumor detection, diagnosis, and monitoring. Here is the latest research on cancer imaging.

© 2022 Meta ULC. All rights reserved