Analysis of the dbpBA upstream regulatory region controlled by RpoS in Borrelia burgdorferi.

Journal of Bacteriology
Zhiming OuyangMichael V Norgard

Abstract

Decorin-binding proteins B and A (DbpB and DbpA) are thought to play important roles in Borrelia burgdorferi pathogenesis by serving as adhesins for the extracellular matrix. It has been established that the expression of DbpBA is governed by the Rrp2-RpoN-RpoS regulatory pathway. However, the precise mechanism underlying the control of DbpBA expression has been unclear. In particular, it has been unknown whether RpoS influences DbpBA expression directly or indirectly (through an additional regulatory molecule[s]). Here, employing a wild-type B. burgdorferi strain and a dbpBA-deficient mutant, we analyzed the 5' genetic elements of the dbpBA operon using deletion analysis, coupled with luciferase reporter assays, quantitative reverse transcription PCR, and immunoblot analyses. A minimal promoter, encompassed within 70 bp upstream of the ATG start codon of dbpBA, was identified and found to be necessary and sufficient to initiate dbpBA transcription. The minimal dbpBA promoter was responsive to environmental stimuli such as temperature, pH, and whole blood. Two in silico-identified inverted repeat elements were not involved in the response of dbpBA expression to in vitro stimulation by environmental factors. The expression of db...Continue Reading

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Citations

Aug 2, 2011·Annual Review of Microbiology·D Scott Samuels
Jun 22, 2016·Infection and Immunity·Zhiming OuyangMichael V Norgard
Jan 24, 2014·Infection and Immunity·Zhiming OuyangMichael V Norgard
May 2, 2019·Frontiers in Cellular and Infection Microbiology·Charlotte MasonZhiming Ouyang
Jun 22, 2017·EcoSal Plus·James T Riordan, Avishek Mitra

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