PMID: 6170934Sep 11, 1981Paper

Analysis of the DNA and RNA changes associated with the expression of isotypic variant-specific antigens of trypanosomes

Nucleic Acids Research
E PaysM Steinert

Abstract

Using specific (32P) labelled cDNA probes, we compared the mRNAs and the genomic DNA sequences coding for the synthesis of two pairs of serologically related variant-specific antigens (VSAs) of trypanosomes: AnTat 1.1 and AnTat 1.1b, both from the strain 1125 of T.b.brucei and AnTat 1.8 and LiTat 1.6 from T.b.brucei and T.b. gambiense, respectively. Within each pair, large similarities were observed in the coding sequence, except in the 3' region which appears to be highly variable. However, a low level of cross-hybridization can be detected between all sequences, in the 3' region only. The expression of these VSAs is linked to a similar duplication-transposition mechanism. The insertion locus of the transposition unit is the same both in AnTat 1.1 and AnTat 1.1b DNAs. In both pairs, the transposition unit seems to comprise at least about 200 bp upstream of the 5' extremity of the coding sequence. The significance of these results, regarding the structure and synthesis of the VSAs, is discussed.

References

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May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·E PaysM Steinert

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Citations

Nov 1, 1983·Molecular and Biochemical Parasitology·M ParsonsN Agabian
Jun 25, 1982·Nucleic Acids Research·L H Van der PloegJ Van Boom
Jul 11, 1985·Nucleic Acids Research·A W CornelissenP Borst
Mar 14, 2000·Molecular and Cellular Biology·K T Militello, L K Read
Apr 5, 1985·Journal of Molecular Biology·A Y LiuP Borst
Jun 1, 1982·Cell·P Borst, G A Cross
Dec 15, 1983·European Journal of Biochemistry·P BorstJ M Kooter
Nov 10, 1983·Nature·J R YoungM J Turner
Feb 1, 1984·The Journal of protozoology·F F Richards
Mar 1, 1987·Molecular and Cellular Biology·B EhlersP Overath
Jun 1, 1985·Microbiological Reviews·J E Donelson, A C Rice-Ficht
Mar 1, 1983·Molecular and Cellular Biology·J D ForneyJ R Preer

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