PMID: 6166391Jun 1, 1981Paper

Analysis of the effect of amines on inhibition of receptor-mediated and fluid-phase pinocytosis in rabbit alveolar macrophages

Cell
J Kaplan, E A Keogh

Abstract

Incubation of rabbit alveolar macrophages in vitro with methyl amine led to a time- and concentration-dependent inhibition of uptake of alpha macroglobulin-125I-trypsin complexes (alpha M-125I-T). Upon addition of methyl amine (50 mM) to cells prelabeled with alpha M-125I-T there was a rapid inhibition of lysosomal catabolism of internalized ligand. In the absence of ligand, incubation of cells with 50 mM methyl amine led to a 40-70% decrease in surface-receptor number. The methyl amine-induced decrease in surface-receptor number only occurred in metabolically active cells since cells incubated at 0 degrees C, or treated with N-ethyl maleimide and incubated at 37 degrees C, did not show the effect. Incubation of cells at 37 degrees C with methyl amine also effected a 40-70% decrease in fluid-phase pinocytosis. Although there was a decline in surface-receptor number, the remaining population of receptors were capable of mediating (at least) one round of ligand internalization. However, further ligand uptake was prevented. Data demonstrate that although receptors were present on cell surfaces, they were incapable of mediating ligand internalization. Incubation of macrophages with chloroquine at 37 degrees C for 60 min also led to...Continue Reading

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Citations

Oct 27, 1998·Journal of Biomedical Materials Research·M WagnerC J Kirkpatrick
Jan 1, 1989·Archives of Microbiology·S DoiM Yoshimura
May 1, 1983·Immunology Letters·M SándorJ Gergely
Mar 1, 1985·Molecular and Biochemical Parasitology·A L Schwartz, M R Hollingdale
Apr 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J M MatoI Pastan
Jun 1, 1987·The Journal of Cell Biology·J E Zijderhand-BleekemolenH J Geuze
Jan 1, 1983·The Journal of Cell Biology·R M SteinmanZ A Cohn
Jun 1, 1983·The Journal of Cell Biology·J HarfordR D Klausner
Sep 1, 1983·The Journal of Cell Biology·D J YamashiroF R Maxfield
Sep 17, 2003·Journal of Experimental Zoology. Part A, Comparative Experimental Biology·Joachim F Schindler
Aug 15, 1989·Biochemical and Biophysical Research Communications·E Omoto, M Tavassoli
Jul 1, 1984·Experimental Cell Research·B GoudJ C Antoine
Mar 1, 1982·Virology·P AndersonJ Vilcek
May 1, 1995·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M HaradaH Yoshida
Jul 1, 1983·Journal of Toxicology and Environmental Health·C AranyiF J Miller
Jan 1, 1983·Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section A, Pathology·J ThybergJ Nilsson
Jan 1, 1982·Annals of the New York Academy of Sciences·R B DicksonI H Pastan
Oct 25, 1983·Biochimica Et Biophysica Acta·A E Solheim, P O Seglen

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