Analysis of the erythropoietin of a Tibetan Plateau schizothoracine fish (Gymnocypris dobula) reveals enhanced cytoprotection function in hypoxic environments

BMC Evolutionary Biology
Qianghua XuLiangbiao Chen

Abstract

Erythropoietin (EPO) is a glycoprotein hormone that plays a principal regulatory role in erythropoiesis and initiates cell homeostatic responses to environmental challenges. The Qinghai-Tibet Plateau is a natural laboratory for hypoxia adaptation. Gymnocypris dobula is a highly specialized plateau schizothoracine fish that is restricted to > 4500 m high-altitude freshwater rivers and ponds in the Qinghai-Tibet Plateau. The role of EPO in the adaptation of schizothoracine fish to hypoxia is unknown. The EPO and EPO receptor genes from G. dobula and four other schizothoracine fish from various altitudinal habitats were characterized. Schizothoracine EPOs are predicted to possess 2-3 N-glycosylation (NGS) sites, 4-5 casein kinase II phosphorylation (CK2) sites, 1-2 protein kinase C (PKC) phosphorylation sites, and four conserved cysteine residues within four helical domains, with variations in the numbers of NGS and CK2 sites in G. dobula. PAML analysis indicated a d N/d S value (ω) = 1.112 in the G. dobula lineage, and a few amino acids potentially under lineage-specific positive selection were detected within the G. dobula EPO. Similarly, EPO receptors of the two high-altitude schizothoracines (G. dobula and Ptychobarbus kaznako...Continue Reading

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Citations

Feb 18, 2017·G3 : Genes - Genomes - Genetics·Dongsheng ZhangLiangbiao Chen
Feb 1, 2020·International Journal of Biological Macromolecules·Chao Tong, Miao Li
Apr 24, 2021·Genome Biology and Evolution·Chao TongKai Zhao
Aug 30, 2021·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Lei XuYuning Hu

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Datasets Mentioned

BETA
AGH20610
ABB83930
AAI62974
AAW29029

Methods Mentioned

BETA
environmental stress
PCR
transfection
Chip
Assay
electrophoresis

Software Mentioned

R
PAUP
BLAST
SPSS
MotifScan
DEseq2
Tophat2
Adaptive MODEL
HTSeq
SignalP

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