PMID: 8588089Nov 1, 1995Paper

Analysis of the expression and secretion of isotypes of sheep B cell immunoglobulins with a panel of isotype-specific monoclonal antibodies

Research in Veterinary Science
P BirdJ Hopkins

Abstract

Monoclonal antibodies to sheep light chain, IgM and IgG were produced and used to assess total immunoglobulin (Ig) synthesis by sheep B cells in culture and antibodies to specific antigens. By using these antibodies in a dual fluorescence-activated cell sorting analysis of sheep efferent lymph B lymphocytes the percentage change in surface Ig isotype of B lymphoblasts from IgM to IgG after the antigenic stimulation of the local lymph node was measured. An extension of this analysis to paired blood and afferent or efferent lymph B cells made it possible to investigate the recirculation characteristics of B cells expressing different Ig isotypes.

References

Apr 1, 1967·Quarterly Journal of Experimental Physiology and Cognate Medical Sciences·J G Hall

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Citations

May 29, 1998·European Journal of Immunology·V K GuptaJ Hopkins
Aug 3, 2014·Research in Veterinary Science·Yongli GuoJunwei Wang
Jul 3, 2010·Preventive Veterinary Medicine·F Rodríguez-CadenasF A Rojo-Vázquez
Apr 14, 2015·Japanese Journal of Infectious Diseases·Shereen HegazyAzza El-Amir
Oct 27, 2001·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·D D CollieH R Miller
Jan 12, 2000·Journal of Dairy Science·D E BaumanR J Collier
Nov 5, 2005·Journal of Applied Physiology·John F PeroniTom P Robertson
Apr 30, 2020·Cell and Tissue Research·Panchan SitthicharoenchaiMark R Ackermann

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