Analysis of the expression of CagA and VacA and the vacuolating activity in 167 isolates from patients with either peptic ulcers or non-ulcer dyspepsia

The American Journal of Gastroenterology
T TakataJ Ono

Abstract

The goals of this study were: 1) to examine the prevalence of cytotoxin-associated protein (CagA), vacuolating cytotoxin (VacA), and the vacuolating cytotoxin activity (VCA) in vitro of infecting Helicobacter pylori isolates and 2) to clarify the relation between the expression of these virulence factors and the occurrence of peptic ulceration. One hundred sixty-seven clinical isolates of H. pylori from patients with peptic ulcer disease (gastric ulcer, 62 cases; duodenal ulcer, 48 cases) and nonulcer dyspepsia (57 cases) were studied regarding their genetic and phenotypic properties. Type 1 bacteria, which had both CagA and VCA, and type 2 bacteria, which did not express either CagA or VCA, represented 62.9% and 7.8%, respectively; the remaining 29.4% had an intermediate phenotype, expressing either CagA independent of the presence of VCA (CagA+VCA-) or vice versa (CagA-VCA+). CagA+VCA- and CagA-VCA+ bacteria represented 17.4 % and 12.0%, respectively, both of which were more numerous than the type 2 category. The proportion of the CagA-positive isolates was significantly higher in both the duodenal ulcer (97.9%) and gastric ulcer (83.9%) patients than in the non-ulcer dyspepsia patients (61.4%) (p < 0.01). On the other hand, ...Continue Reading

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Citations

Jun 9, 2005·Srpski arhiv za celokupno lekarstvo·Aleksandra Sokić-MilutinovićTomica Milosavljević
Dec 6, 2007·Helicobacter·Barry Marshall, Tobias Schoep
Oct 13, 2000·Gastroenterology Clinics of North America·M F Go, S E Crowe
May 24, 2005·The Journal of Infection·Motonobu MiyazakiHiroshi Une
Oct 17, 2007·Digestive Diseases and Sciences·Yeganeh TalebkhanLeili Zamaninia
Oct 4, 2002·European Journal of Gastroenterology & Hepatology·Gonzalo Castillo-RojasYolanda López-Vidal
Mar 30, 2000·Microscopy Research and Technique·G Faller, T Kirchner
Jul 11, 2003·World Journal of Gastroenterology : WJG·Kantarceken BulentHilmioglu Fatih
Jun 23, 1999·FEMS Immunology and Medical Microbiology·Y Akyön, G Hascelik
Aug 15, 1998·Journal of Clinical Microbiology·L J van DoornW G Quint
Jun 12, 1999·Journal of Clinical Microbiology·L J van DoornW G Quint

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