Analysis of the expression of NB1 antigen using two monoclonal antibodies

Transfusion
D F StroncekL T Clement

Abstract

Neutrophil-specific antigen NB1 is expressed on neutrophil subpopulations in 97 percent of healthy individuals and is located on 56- to 64-kDa glycoprotein. While the molecule carrying NB1 has been identified, the nature of the NB1 epitope has not been well characterized. Two monoclonal antibodies (MoAbs), 1B5 and the recently produced 7D8, and four alloantibodies, all specific for NB1, were used to investigate the expression of NB1 on neutrophils from several donors. MoAb 7D8 was shown to be specific for NB1. It reacted with NB1-positive neutrophils from 52 donors in the granulocyte immunofluorescence assay and did not react with NB1-negative neutrophils from 8 donors. MoAb 7D8 immunoblotted a 56- to 64-kDa molecule on neutrophils from eight NB1-positive donors and did not react with this molecule on NB1-negative neutrophils from two donors. When 7D8 was tested in the monoclonal antibody immobilization of granulocyte antigens assay, it reacted with two NB1 alloantibodies, but not with NA1 or NA2 alloantibodies. To determine if MoAbs 7D8 and 1B5 recognized the same epitope, both were tested against the same NB1-positive neutrophils and the cells were analyzed by two-color flow cytometry. Both antibodies bound independently to n...Continue Reading

Citations

Oct 26, 1999·Transfusion·H ChunD F Stroncek
Jun 24, 2000·Transfusion·K MatsuoD Stroncek
Mar 31, 2004·Journal of Translational Medicine·David F StroncekMaria Bettinotti
Jun 15, 2010·Life Sciences·Jamie R PrivratskyPeter J Newman
Nov 3, 2007·Experimental Hematology·Jaroslav JelinekVahid Afshar-Kharghan
Apr 5, 2003·Transfusion·Lorraine CaruccioDavid Stroncek
Feb 16, 2002·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Maria P BettinottiDavid Stroncek
Oct 14, 2016·JCI Insight·Jennifer K RoeMahdad Noursadeghi
Oct 15, 2013·American Journal of Clinical Pathology·Howard J MeyersonAnna Balog
Apr 24, 2018·European Journal of Clinical Investigation·Pien HellebrekersLeo Koenderman
Jun 21, 2007·The Journal of Biological Chemistry·Ulrich J H SachsSentot Santoso
Mar 11, 2021·Cellular and Molecular Gastroenterology and Hepatology·Broc DruryGwo-Tzer Ho

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