Aug 5, 2008

Analysis of the expression of putatively imprinted genes in bovine peri-implantation embryos

Theriogenology
P Tveden-NyborgN T D'Cruz

Abstract

The application of assisted reproductive technologies (ART) has been shown to induce changes in the methylation of the embryonic genome, leading to aberrant gene expression, including that of imprinted genes. Aberrant methylation and gene expression has been linked to the large offspring syndrome (LOS) in bovine embryos resulting in increased embryonic morbidity and mortality. In the bovine, limited numbers of imprinted genes have been studied and studies have primarily been restricted to pre-implantation stages. This study reports original data on the expression pattern of 8 putatively imprinted genes (Ata3, Dlk1, Gnas, Grb10, Magel2, Mest-1, Ndn and Sgce) in bovine peri-implantation embryos. Two embryonic developmental stages were examined, Day 14 and Day 21. The gene expression pattern of single embryos was recorded for in vivo, in vitro produced (IVP) and parthenogenetic embryos. The IVP embryos allow us to estimate the effect of in vitro procedures and the analysis of parthenogenetic embryos provides provisional information on maternal genomic imprinting. Among the 8 genes investigated, only Mest-1 showed differential expression in Day 21 parthenogenetic embryos compared to in vivo and IVP counterparts, indicating maternal...Continue Reading

Mentioned in this Paper

Pathologic Cytolysis
Embryo
Polymerase
Morbidity Aspects
Tissue Membrane
Heparin
GNAS gene
Benign Mixed Epithelial and Stromal Tumor of Kidney
Protein Methylation
Placenta Specimen

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