PMID: 16627954Apr 22, 2006Paper

Analysis of the function of activin betaC subunit using recombinant protein

The Journal of Reproduction and Development
Yuuki UshiroYoshihisa Hasegawa

Abstract

Activins, TGF-beta superfamily members, have multiple functions in a variety of cells and tissues. Additional activin beta subunit genes, betaC and betaE, have been identified in humans and rodents. To explore the role of activin betaC subunit, we generated recombinant human activin C using Chinese hamster ovary cells. Recombinant activin C from the conditioned medium was purified by consecutive hydrophobic, size-exclusion, and high performance liquid chromatography. SDS-PAGE and Western blot analysis of the purified protein revealed that activin C formed disulfide bridges. However, activin C had no effect on the proliferation of cultured liver cells. Furthermore, there were no significant differences in erythroid differentiation and follicle stimulating hormone secretion in vitro. It was also shown that immunoreactive bands indicated the hetrodimer of activin betaC, and inhibin alpha subunits were detected in the conditioned medium from the activin C-producing cells, which were stably transfected with inhibin alpha subunit cDNA. This suggests that activin betaC subunit may have been present and that it may exert its effect as inhibin C.

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Citations

Mar 20, 2008·World Journal of Gastroenterology : WJG·Alev DeliMichael Grusch
May 12, 2010·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Tobias WeissenbacherIoannis Mylonas
Nov 26, 2010·Reproductive Biology and Endocrinology : RB&E·Ioannis MylonasMarkus S Kupka
Jan 26, 2012·Brain : a Journal of Neurology·Xing-Jun LiuXu Zhang
Sep 26, 2006·Mutation Research·Chantal Rodgarkia-DaraMichael Grusch
Oct 31, 2009·World Journal of Hepatology·Emanuel KreidlMichael Grusch
Oct 29, 2015·Molecular Carcinogenesis·Xiuli BiWancai Yang
Dec 2, 2010·Systems Biology in Reproductive Medicine·Ioannis MylonasBarbara Schiessl
Jun 23, 2016·Cold Spring Harbor Perspectives in Biology·Maria Namwanje, Chester W Brown

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