PMID: 9188557Jul 1, 1997Paper

Analysis of the gene start and gene end signals of human respiratory syncytial virus: quasi-templated initiation at position 1 of the encoded mRNA

Journal of Virology
L KuoP L Collins

Abstract

The gene start (GS) and gene end (GE) transcription signals of human respiratory syncytial virus (RSV) strain A2 were analyzed in helper-dependent monocistronic and dicistronic minireplicons which were complemented by a standard RSV strain. The GS signal, which is the start site for mRNA synthesis, is highly conserved for the first nine genes: 3'-CCCCGUUUA(U/C) (negative sense). This conserved version of the signal was analyzed by "saturation" mutagenesis, in which all 10 positions, as well as one downstream and one upstream position, were changed one at a time into each of the other three nucleotides. Most of the positions appear to contribute to the signal: positions 1, 3, 6, 7, and, in particular, 9 were the most sensitive, whereas position 5 was relatively insensitive. The effect of nucleotide substitution in the first position of the signal was examined further by cDNA cloning and sequence analysis of the residual mRNA which was produced. For the two mutants examined (1C to U, and 1C to A), the site of initiation was unchanged. However, the mRNAs were dimorphic with regard to the assignment of the 5'-terminal nucleotide: two-thirds contained the predicted mutant substitution, and one-third contained the parental assignment...Continue Reading

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Citations

Sep 26, 2002·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Arshud M DarVivek Kapur
Jan 20, 2004·The Pediatric Infectious Disease Journal·Gail W Wertz, Robin M Moudy
Jan 30, 2007·Veterinary Research·Jean-Francois Valarcher, Geraldine Taylor
Jun 1, 2007·Journal of Virology·Nancy BeerensBruno Canard
May 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Sarah L NotonRachel Fearns
Aug 10, 2007·Journal of Virology·Jennifer T WangSean P J Whelan
Apr 16, 2004·Clinical Microbiology Reviews·Andrew J EastonHelene F Rosenberg
Nov 22, 2005·The Journal of General Virology·Nicole L Edworthy, Andrew J Easton
Oct 22, 2002·The Journal of General Virology·Gabriele NeumannYoshihiro Kawaoka
Aug 17, 2006·FEMS Microbiology Reviews·Reena GhildyalDavid A Jans
May 21, 2020·Viral Immunology·Dongdong Cao, Bo Liang
Jun 9, 2020·Frontiers in Cellular and Infection Microbiology·Elena Margaret Thornhill, David Verhoeven
Sep 28, 2016·Virology Journal·Shadi ShahriariReena Ghildyal
Jan 11, 2020·PloS One·Felipe-Andrés PiedraPedro A Piedra
Oct 27, 2017·Viruses·Alex HarwigBen Berkhout
Dec 16, 1998·Journal of Virology·R Fearns, P L Collins
Aug 28, 2020·Journal of Virology·Bo Liang
Jun 7, 2018·Nucleic Acids Research·Tessa N CresseyRachel Fearns
Jun 9, 2006·The Journal of General Virology·Vanessa M CowtonRachel Fearns

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