Analysis of the genetic determinant for production of the peptide antibiotic nisin

Journal of General Microbiology
H M DoddM J Gasson

Abstract

The structural gene for the precursor of the peptide antibiotic nisin was isolated and characterized. As with other lanthionine-containing antibiotics, nisin is synthesized as a pre-propeptide which undergoes post-translational modification to generate the mature antibiotic. The sequence data obtained agreed with those of precursor nisin genes isolated by other workers from different Lactococcus lactis strains. Analysis of regions flanking the precursor nisin gene revealed the presence of a downstream open reading frame that may be involved in maturation of the precursor molecule. Nucleotide sequences characteristic of an IS element were located upstream of the nisin determinant. This element, termed IS904, is present in multiple copies in the genome of L. lactis. The nisin determinant of L. lactis is a component of a large transmissible gene block that also encodes nisin resistance and sucrose-metabolizing genes. Gene probe experiments indicated that the nisin/sucrose gene block was located in the chromosome. Furthermore, the copy of IS904 identified adjacent to the precursor nisin gene lies at, or very close to, one end of this transmissible DNA segment and may play a role in mediating its transfer between strains.

Citations

Sep 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R JackH G Sahl
Dec 1, 1994·Biodegradation·R C WyndhamM C Peel
Jan 1, 1995·Journal of Tongji Medical University = Tong Ji Yi Ke Da Xue Xue Bao·S Y YeP E Saris
Jul 1, 1996·International Journal of Food Microbiology·R Geisen, W H Holzapfel
Sep 1, 1993·FEMS Microbiology Reviews·P Le BourgeoisP Ritzenthaler
Apr 23, 2003·Biochemical and Biophysical Research Communications·Carsten CorveyKarl-Dieter Entian
Oct 17, 2002·International Journal of Food Microbiology·J M RodríguezH M Dodd
Oct 18, 2002·International Journal of Food Microbiology·R Paul RossC Hill
Nov 30, 2002·International Journal of Food Microbiology·W NoonpakdeeS Panyim
Jan 14, 2000·International Journal of Food Microbiology·N A OlasupoW H Holzapfel
May 12, 2001·FEMS Microbiology Reviews·O McAuliffeC Hill
Apr 26, 2002·Molecular Microbiology·Torsten SteinKarl-Dieter Entian
Apr 24, 1999·European Journal of Biochemistry·A Karakas SenM J Gasson
Jan 1, 1994·Critical Reviews in Food Science and Nutrition·J N Hansen
Jul 25, 1990·Nucleic Acids Research·P J RauchW M de Vos
Mar 25, 1992·Nucleic Acids Research
Feb 1, 1995·The Journal of Applied Bacteriology·J M RodríguezM J Gasson
Jun 1, 1996·The Journal of Applied Bacteriology·M QiaoP E Saris
Nov 1, 1991·European Journal of Biochemistry·J W MuldersW M de Vos
Feb 15, 1992·European Journal of Biochemistry·N SchnellK D Entian
Jun 29, 2002·Applied and Environmental Microbiology·Haiping Li, Daniel J O'Sullivan
Aug 6, 2004·Applied and Environmental Microbiology·Nikki HornJuan M Rodríguez
Nov 18, 2004·Journal of Bacteriology·Anja H SchiemannPaul W O'Toole
Oct 20, 2005·Journal of Bioscience and Bioengineering·C LosteinkitS Shioya
Feb 1, 1996·Antonie van Leeuwenhoek·K D Entian, W M de Vos
Feb 1, 1996·Antonie van Leeuwenhoek·P E SarisH G Sahl
Feb 1, 1996·Antonie van Leeuwenhoek·R J SiezenW M de Vos
Oct 1, 1996·Antonie van Leeuwenhoek·B E DavidsonA J Hillier
Feb 1, 1993·Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology·G Bierbaum, H G Sahl
Mar 8, 2003·Molecular Microbiology·Torsten SteinKarl-Dieter Entian
Nov 14, 2006·FEMS Microbiology Reviews·Alain DufourJean-Paul Le Pennec
Aug 11, 2010·FEMS Microbiology Reviews·Hikmate AbriouelAntonio Gálvez
Feb 1, 1992·FEMS Microbiology Reviews·M van de GuchteG Venema

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