PMID: 9663853Jul 15, 1998Paper

Analysis of the IgG autoantibody repertoire in endocrine ophthalmopathy using the MegaBlot technique

Current Eye Research
F H Grus, A J Augustin

Abstract

Normal sera contain a large number of naturally-occurring autoantibodies which can complicate the differentiation of disease-associated autoantibodies from the complex background of "autoimmune noise" (i.e. naturally-occurring autoantibodies). The aim of this study was to analyze the human IgG autoantibody repertoire of sera from patients suffering from endocrine ophthalmopathy and to compare those to sera from healthy subjects using the recently developed MegaBlot technique. This new method allows the simultaneous global and quantitative screening for reactivities of antibodies with a large number of antigens. Sera (n = 17: endocrine ophthalmopathy; n = 10: healthy subjects) were tested against Western blots of SDS-PAGE preparations of proteins from human extraorbital eye muscle. Digital image analysis was performed (ScanPacK, Biometra, Germany). The blots were subsequently analyzed by our new MegaBlot technique. Both the sera of patients suffering from endocrine ophthalmopathy and the sera of healthy subjects revealed reactivities of antibodies against eye muscle proteins (up to 15 bands/blot). The MegaBlot procedure could differentiate between the banding patterns of patients suffering from endocrine ophthalmopathy and healt...Continue Reading

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Citations

Apr 13, 2004·Movement Disorders : Official Journal of the Movement Disorder Society·Harvey S SingerFranz H Grus

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