Analysis of the Impact of Common Polymorphisms of the FTO and MC4R Genes with the Risk of Severe Obesity in Saudi Arabian Population

Genetic Testing and Molecular Biomarkers
Cyril CyrusAmein Al Ali

Abstract

Obesity has reached epidemic proportions worldwide resulting in a serious public health problem. In Saudi Arabia, 28.7% of the population is obese due largely to the adoption of western dietary patterns over the last decade. The fat-mass and obesity associated (FTO) and melanocortin-4 receptor (MC4R) genes are ubiquitously expressed in the brain and pancreatic islets, and are the main link between the central nervous system and energy homeostasis based on food intake and energy expenditure. Genetic variants in the FTO and MC4R genes have been strongly associated with an increased obesity risk. To identify novel mutations in the MC4R gene and to perform correlation analyses of the known variants rs9939609 and rs1421085 in the FTO gene and rs17782313 near the MC4R gene in an obese Saudi population. A total of 136 obese patients and 104 healthy controls from King Fahd Hospital, Al-Khobar, were genotyped for single-nucleotide polymorphisms within or near the FTO and MC4R genes using the TaqMan assay. Leptin levels were determined by enzyme-linked immunosorbent assay. Targeted sequencing of MC4R exon was done by Sanger sequencing method. The study included 58 obese males and 78 obese females with a mean age of 39.78 ± 12.77 years an...Continue Reading

References

May 29, 2002·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·E Miraglia Del GiudiceL Perrone
Mar 21, 2003·The New England Journal of Medicine·I Sadaf FarooqiStephen O'Rahilly
Apr 28, 2004·Diabetes·William A BanksJohn E Morley
Oct 29, 2005·Nature·UNKNOWN International HapMap Consortium
Aug 24, 2006·The New England Journal of Medicine·Kenneth F AdamsMichael F Leitzmann
May 15, 2007·Nature Genetics·Christian DinaPhilippe Froguel
Oct 19, 2007·Nature·UNKNOWN International HapMap ConsortiumJohn Stewart
Dec 7, 2007·Molecular Genetics and Metabolism·Armand PeetersWim Van Hul
Feb 2, 2008·Obesity·Steven C HuntTed D Adams
May 6, 2008·Nature Genetics·Ruth J F LoosKaren L Mohlke
Jan 21, 2009·International Journal of Obesity : Journal of the International Association for the Study of Obesity·F StutzmannP Froguel
Oct 14, 2009·Evidence-based Complementary and Alternative Medicine : ECAM·Seongwon ChaJong Y Kim
Oct 12, 2010·Nature Genetics·Elizabeth K SpeliotesRuth J F Loos
Dec 28, 2010·Nutrition, Metabolism, and Cardiovascular Diseases : NMCD·D CorellaJ M Ordovas
Feb 8, 2011·European Journal of Pharmacology·Ruth J F Loos
Jun 10, 2011·BMC Medicine·Sihua PengMaode Lai
Nov 7, 2012·Nature·UNKNOWN 1000 Genomes Project ConsortiumGil A McVean
Jan 31, 2013·Annals of the New York Academy of Sciences·Qianghua Xia, Struan F A Grant
May 29, 2013·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Christine PodriniJacqueline K White
Feb 13, 2015·Nature·Adam E LockeElizabeth K Speliotes
Jan 18, 2016·Cancer Epidemiology·Melina ArnoldIsabelle Soerjomataram

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Citations

Feb 16, 2019·Archives of Physiology and Biochemistry·Ehab M M AliAhmad Settin
Jan 4, 2020·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Gyeong Im YuDong Hoon Shin
Apr 3, 2020·Nature Reviews. Endocrinology·Rodrigo San-CristobalJosé Alfredo Martínez
Jun 17, 2021·International Journal of Obesity : Journal of the International Association for the Study of Obesity·Salma YounesHatem Zayed

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Methods Mentioned

BETA
enzyme-linked
ELISA
genotyping
PCR
electrophoresis

Software Mentioned

SPSS
Applied
Codon code analyzer

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