Analysis of the inhibitory effect of inorganic phosphate on development of four-cell hamster embryos in vitro

The Journal of Experimental Zoology
H Monis, B D Bavister

Abstract

Hamster 4-cell stage embryos were cultured in a protein-free, glucose-free medium to study the nature of developmental inhibition by inorganic phosphate (Pi). In the absence of Pi, between 40 and 55% of embryos were able to develop to the blastocyst stage but addition of Pi to the medium reduced this proportion to 5-20%. The inhibition did not appear to be due to contamination of the Pi salt with heavy metals because EDTA did not relieve the effect. Inhibition by Pi showed no dose-response relationship over the range tested (1-350 microM). In contrast, another divalent anion (sulphate) produced no inhibition of 4-cell embryo development at concentrations as high as 5.6 mM. Embryos were less sensitive to inhibition by Pi after the third cleavage division had occurred, and development of mid or late 8-cell embryos was unaffected by Pi. After exposure to Pi for 1 h, embryos could recover and continue development but longer exposure was detrimental to subsequent development. These results indicate that the inhibitory effect is specific to phosphate ions, is not due to contaminants in the Pi salt, is evoked by very low concentrations of Pi, is stage-specific, and is reversible following brief exposure of embryos to Pi. These effects...Continue Reading

References

Dec 1, 1987·Journal of in Vitro Fertilization and Embryo Transfer : IVF·S Lee
Mar 1, 1989·Biology of Reproduction·P B Seshagiri, B D Bavister
Mar 1, 1989·Biology of Reproduction·P B Seshagiri, B D Bavister
Jul 1, 1989·Journal of Reproduction and Fertility·C L ChatotI Torres
Dec 1, 1988·Fertility and Sterility·B FischerH M Beier
Dec 1, 1988·Biology of Reproduction·S A Schini, B D Bavister
Apr 1, 1988·Journal of in Vitro Fertilization and Embryo Transfer : IVF·B D Bavister, J C Andrews
Feb 1, 1983·Biology of Reproduction·B D BavisterG Lieberman
Mar 1, 1981·Journal of Reproduction and Fertility·G A SchultzM H Johnson
Jan 1, 1929·The Biochemical Journal·H G Crabtree

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Citations

May 1, 1992·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·B FischerC Hegele-Hartung
Feb 1, 1997·Zygote : the Biology of Gametes and Early Embryos·K Miyoshi, K Niwa
Feb 18, 2004·The Journal of Reproduction and Development·Myung-Sook Han, Koji Niwa
Apr 25, 2001·Biology of Reproduction·T E LudwigB D Bavister
Jun 29, 2005·Journal of Personality and Social Psychology·Wendy WoodLeona Tam
May 11, 2002·Journal of Personality and Social Psychology·Paul D WindschitlAnnette R Flugstad
May 11, 2002·Journal of Personality and Social Psychology·Patricia G DevineStephanie L Vance
Dec 26, 2002·Journal of Personality and Social Psychology·Boris Egloff, Stefan C Schmukle
Aug 2, 2002·Journal of Personality and Social Psychology·Jens B AsendorpfDaniel Mücke
May 4, 2002·Cultural Diversity & Ethnic Minority Psychology·John F DovidioGordon Hodson
Nov 17, 2005·Journal of Personality and Social Psychology·Frederica R ConreyCarla J Groom
Aug 3, 2006·Journal of Personality and Social Psychology·Nilanjana Dasgupta, Luis M Rivera
Aug 17, 2006·Psychological Bulletin·Laura R Glasman, Dolores Albarracín

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