Analysis of the interaction between the UL11 and UL16 tegument proteins of herpes simplex virus.

Journal of Virology
Pei-Chun YehJohn W Wills

Abstract

The UL11 and UL16 tegument proteins of herpes simplex virus are conserved throughout the herpesvirus family. Previous studies have shown that these proteins interact, perhaps to link UL16-bound nucleocapsids to UL11, which resides on the cytoplasmic face of the trans-Golgi network, where maturation budding occurs. Little is known about the interaction except that it requires the leucine-isoleucine (LI) and acidic cluster motifs in UL11 and that no other viral proteins are involved. In particular, the important question of whether these two proteins bind to each other directly has not been addressed. Accordingly, UL11 and UL16 were expressed in bacteria, and the purified proteins were found to retain the ability to interact in a manner that was dependent upon the LI and acidic cluster. In an attempt to map the UL11-binding site contained in UL16, a large number of deletion mutants were constructed. The first 40 (nonconserved) amino acids were found to be dispensable, but all the other constructs failed to bind UL11 or had poor expression in transfected cells, suggesting that UL16 is very sensitive to alterations and probably lacks a multidomain structure. As an alternative strategy for identifying residues that are important for...Continue Reading

References

Dec 1, 1989·The Journal of General Virology·C A MacLeanD J McGeoch
Mar 19, 1996·Proceedings of the National Academy of Sciences of the United States of America·F M Boyce, N L Bucher
Jun 30, 1998·Archives of Virology·S OshimaY Nishiyama
Jan 19, 2002·Journal of Virology·Thomas C Mettenleiter
Jun 28, 2002·Cell·Tom Kirchhausen
Mar 1, 1964·Proceedings of the Society for Experimental Biology and Medicine·K O SMITH
Oct 15, 2003·Journal of Virology·Joshua S LoomisJohn W Wills
Nov 30, 2004·Virus Research·Thomas C Mettenleiter
Jul 15, 2005·Journal of Virology·Valerio VittoneRussell J Diefenbach
Aug 25, 2006·Journal of Virology·Joshua S LoomisJohn W Wills
Sep 16, 2006·Journal of Virology·Gaudeline Rémillard-LabrosseRoger Lippé
Sep 15, 2007·Journal of Virology·David G Meckes, John W Wills
Aug 22, 2008·Journal of Virology·David G Meckes, John W Wills

❮ Previous
Next ❯

Citations

Jul 1, 2011·Journal of Virology·Silke ManingerZsolt Ruzsics
Jun 6, 2009·Journal of Virology·William W Newcomb, Jay C Brown
Aug 22, 2008·Journal of Virology·David G Meckes, John W Wills
Aug 24, 2012·Journal of Virology·Pooja ChadhaJohn W Wills
Jan 1, 2010·Journal of Virology·Amy L HarperJohn W Wills
Mar 15, 2013·Journal of Virology·Valerie Le SageBruce W Banfield
Aug 25, 2011·Virology Journal·Qin HeXiaoyue Chen
Nov 15, 2013·Journal of Virology·Moriah L SzparaAndrew J Davison
Sep 24, 2015·Viruses·Danielle J OwenStephen C Graham
Jul 21, 2009·Virus Research·Barbara J KellyRussell J Diefenbach
Aug 5, 2009·Virus Research·Thomas C MettenleiterHarald Granzow
Feb 5, 2013·Journal of Virological Methods·Qin HeXiaoyue Chen
Jul 8, 2016·Molecular & Cellular Proteomics : MCP·Paul AshfordMaya Topf
Oct 3, 2019·MSystems·Anna Hernández DuránMaya Topf
Dec 15, 2010·Protein & Cell·Haitao GuoHongyu Deng
Jan 10, 2020·BMC Veterinary Research·Linjiang YangXiaoyue Chen
Aug 23, 2020·International Journal of Molecular Sciences·Imran Ahmad, Duncan W Wilson
Dec 31, 2020·Viruses·Christos DogrammatzisMaria Kalamvoki
Apr 10, 2021·Virology·Grayson DuRaine, David C Johnson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.