PMID: 8948487Nov 14, 1996Paper

Analysis of the interaction of an anti-HIV peptide, T22 ([Tyr5, 12, Lys7]-polyphemusin II), with gp120 and CD4 by surface plasmon resonance

Biochimica Et Biophysica Acta
H TamamuraN Fujii

Abstract

We have previously found that T22 ([Tyr5, 12, Lys7]-polyphemusin II) exhibits strong anti-human immunodeficiency virus (HIV) activity comparable to that of 3'-azido-2', 3'-dideoxythymidine (AZT). The inhibition mechanism of T22 on HIV-replication has not been elucidated precisely yet, and hence the target molecules of T22 have not been identified. However, our recent research suggested that T22 exerts its effect by blocking virus-cell fusion at an early stage of HIV infection and that T22 might interact with an HIV envelope protein and/or a T-cell surface protein, both of which are critical for HIV infection. In this paper we demonstrated that T22 binds specifically to both gp120 (an envelope protein of HIV) and CD4 (a T-cell surface protein) and that both bindings can be inhibited by an anti-T22 antibody, using biosensor technology (BIAcoreTM) based on the principles of surface plasmon resonance. Linearization by the BIAcoreTM system (BIAlogue software) and nonlinear least squares analysis by curve fitting with exponential equations showed that both interactions have close dissociation constants (approximately 10(-7) M). The present study suggests that T22 inhibits the virus-cell fusion process through binding to both gp120 an...Continue Reading

References

Dec 15, 1992·Biochemical and Biophysical Research Communications·M MasudaT Murakami
Oct 13, 1995·Biochemical and Biophysical Research Communications·B S WeeksN Fujii
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·G PayneC T Walsh
Dec 30, 1994·Biochemical and Biophysical Research Communications·H TamamuraN Yamamoto
Jul 15, 1994·Biochemical and Biophysical Research Communications·B S WeeksN Fujii
May 1, 1993·Chemical & Pharmaceutical Bulletin·H TamamuraN Fujii

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Citations

Mar 21, 1998·Current Opinion in Biotechnology·M FivashR J Fisher
Jul 19, 2006·Clinical Microbiology Reviews·Håvard JenssenRobert E W Hancock
Jun 19, 2001·Biological Chemistry·W van 't HofA V Amerongen
May 20, 1999·Biopolymers·D Andreu, L Rivas
Nov 30, 2013·Pharmaceuticals·Ali Adem Bahar, Dacheng Ren
Apr 21, 2012·Organic & Biomolecular Chemistry·Shinya Oishi, Nobutaka Fujii
Apr 23, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Wei WangRobert I Lehrer
Feb 28, 2019·Frontiers in Immunology·Vincent LarochetteYves Delneste
Aug 16, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Siyu ZhuZiwei Huang
Jan 27, 1999·Biochemical and Biophysical Research Communications·H TamamuraN Fujii

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