PMID: 2493055Feb 1, 1989Paper

Analysis of the interaction of human C5a and C5a des Arg with human monocytes and neutrophils: flow cytometric and chemotaxis studies

The Journal of Investigative Dermatology
K B YanceyL Harvath

Abstract

C5a and C5a des Arg are potent complement-derived mediators that bind receptors on peripheral blood leukocytes and tissue-specific cellular elements to elicit and amplify inflammatory and immunomodulatory reactions. To study the interactions of C5a and C5a des Arg with these cells, fluorescein conjugates of these ligands were prepared by a new technique and their binding to monocytes, neutrophils, platelets, and endothelial cells was studied with flow cytometry. Fluoresceinated C5a produced neutrophil myeloperoxidase release and chemotaxis and also bound rabbit anti-C5a antibody much like native anaphylatoxin; likewise, fluoresceinated C5a des Arg demonstrated retention of biologic and antigenic activities. Both fluorescein-conjugated C5a and C5a des Arg bound to monocytes and neutrophils in a concentration-dependent, saturable, and homogeneous manner, but 10- to 15-fold higher concentrations of C5a des Arg were required to attain saturable binding of these leukocytes. Ligand binding was specifically inhibited by native purified human C5a in a concentration-dependent manner, while it was unaffected by C3a or N-formyl-methionyl-leucyl-phenylalanine-lysine. There was no evidence of a C5a receptor-negative subpopulation of monocyt...Continue Reading

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Citations

Feb 13, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·S M DrouinR A Wetsel
Jan 1, 2004·American Journal of Respiratory and Critical Care Medicine·Jung-Won ParkErwin W Gelfand
Apr 4, 2021·Cancers·Rebecca M O'BrienNiamh Lynam-Lennon
Apr 29, 1999·Clinical Immunology : the Official Journal of the Clinical Immunology Society·D J BirminghamA A Creasey
Jun 17, 1998·Journal of Neuroimmunology·F LahrtzA Fontana
Apr 28, 1994·Biochimica Et Biophysica Acta·P N MonkL J Partridge

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