Analysis of the interdependent localization of vimentin and microtubules in neoplastic myoepithelial cells

Cell Motility and the Cytoskeleton
R G JaegerB Kachar

Abstract

Salivary gland neoplastic myoepithelial cells in culture form very thin cytoplasmic processes in which the vimentin network is well dispersed. These vimentin filaments can be individually visualized by immunofluorescence. In this study, we have analyzed the role of microtubules in the distension and organization of the vimentin filament network found in these cells. We find that vimentin filaments colocalize along microtubules; however, a significant number of filaments can also be found in microtubule-free domains. Additionally, vimentin filaments are absent from large domains of microtubule-rich domains. Treatment of neoplastic myepithelial cells with the microtubule inhibitor nocodazole did not cause any retraction of the distended vimentin network. This observation suggests that the structural integrity of microtubules is not important for the stability of the vimentin network. Combining procedures for transient disruption of vimentin filaments and microtubules we observed that, in the absence of microtubules, the vimentin network could reassemble in the perinuclear region but was unable to extend toward the cell periphery. The dispersion of vimentin filaments to the peripheral regions of the cytoplasm could only be observe...Continue Reading

References

Dec 1, 1978·The Journal of Cell Biology·E Wang, R D Goldman
Jan 1, 1991·Cell Motility and the Cytoskeleton·O Skalli, R D Goldman
Jan 1, 1990·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·V C de Araújo, N S de Araújo
Feb 23, 1990·Cell·P M Steinert, R K Liem
Jan 1, 1985·Annual Review of Cell Biology·P M Steinert, D A Parry
Jan 1, 1988·Annual Review of Biochemistry·P M Steinert, D R Roop
Jan 1, 1988·Experimental Cell Research·M W Klymkowsky
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D Z FisherG Blobel
Oct 1, 1986·The Journal of Cell Biology·M TerasakiK Fujiwara
Oct 1, 1969·Experimental Cell Research·R D Goldman, E A Follett
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·P M SteinertB A Dale
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·F C RamaekersG P Vooijs
Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·E H Ball, S J Singer
Aug 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·B Geiger, S J Singer

❮ Previous
Next ❯

Citations

Jul 1, 2008·Journal of Oral Science·Sueli P H MiyagiMárcia M Marques
Jan 16, 2007·International Journal of Experimental Pathology·Márcia M MarquesCristiane M França
Feb 12, 1998·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·R G JaegerV C de Araújo
Nov 26, 2013·Biomaterials·Shagufta H ShabbirMilan Mrksich

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica
K EdvardsenN Brünner
Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery
R L CarrauS P Cass
Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology
R MunakataT Saku
© 2021 Meta ULC. All rights reserved