Analysis of the intestinal microflora in Hepialus gonggaensis larvae Using 16S rRNA sequences

Current Microbiology
Hewei YuYouping Yin

Abstract

Gut microbial diversity provides insight into the basic function of a gut microbial ecosystem. In this study, restriction fragment length polymorphism 16S rRNA sequences was used to detect the intestinal microbial diversity of Hepialus gonggaensis larvae. The total DNA of microorganisms was extracted from the intestinal contents and 16S rRNA was amplified. A nearly full-length of 16S rRNA sequence library was constructed. The fingerprints of the microorganisms were analyzed by isolating plasmid and then digesting them with EcoRI, MspI, and HaeIII enzymes, respectively. The library established includes 35 restriction endonuclease types and a phylogenetic tree depicted the linkage of the isolated microbial from the guts of H. gonggaensis larvae. The dominant bacteria in the guts of H. gonggaensis larvae belong to Rahnella sp and Carnobacterium sp and accounted for 45.58% and 30.88% of the total 16S rRNA clones library, respectively. The result showed that bacteria diversity in the guts of H. gonggaensis larvae had some differences from those isolated from normal environment.

References

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Jan 24, 2007·Comparative Immunology, Microbiology and Infectious Diseases·José Luis BalcázarJosé Luis Muzquiz

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Citations

Jan 27, 2011·Current Microbiology·Hyunbum JeonSung-Jae Lee
Feb 23, 2010·BMC Microbiology·Wilfried RozhonBrigitte Poppenberger
Jun 3, 2014·BMC Microbiology·Marcello TagliaviaPaola Quatrini
May 15, 2012·Journal of Basic Microbiology·Ali SevimFiliz Ozkan
Sep 18, 2012·Molecular Ecology·D R ColmanC D Takacs-Vesbach

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