Analysis of the Legionella pneumophila fliI gene: intracellular growth of a defined mutant defective for flagellum biosynthesis.

Infection and Immunity
J J MerriamR R Isberg

Abstract

Using a PCR-based strategy and degenerate oligonucleotides, we isolated a Legionella pneumophila gene that showed high sequence similarity to members of the fliI gene family. An insertion mutation that disrupted the fliI open reading frame was recombined onto the L. pneumophila chromosome and analyzed for its effects on production of flagella and intracellular growth. The mutation resulted in loss of surface-localized flagellin protein but had no effect on the ability of the bacteria to grow within cultured cells. Therefore, in spite of the fact that some aflagellar mutations render L. pneumophila unable to grow within macrophages, the isolation of this defined mutant confirms that production of flagella is not required for intracellular growth.

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Citations

Jul 12, 2002·Microscopy Research and Technique·Iouri F Belyi
Oct 17, 2006·Molecular Microbiology·Carr D VincentJoseph P Vogel
Oct 24, 2007·Nature·Alyssa IngmundsonCraig R Roy
Aug 30, 2011·Proceedings of the National Academy of Sciences of the United States of America·Tamara J O'ConnorRalph R Isberg
Jun 20, 2014·PLoS Pathogens·Halie K MillerVictoria Auerbuch
Oct 6, 2000·Annual Review of Microbiology·M S Swanson, B K Hammer
Apr 23, 2002·Molecular Microbiology·Brian K HammerMichele S Swanson
Jun 19, 2004·Journal of Bacteriology·Kimberly A Walker, Virginia L Miller
Apr 16, 2004·Clinical Microbiology Reviews·Gilbert Greub, Didier Raoult
Apr 12, 2006·The Journal of Experimental Medicine·Ari B MolofskyMichele S Swanson
Sep 10, 2005·Applied and Environmental Microbiology·Michelle E Maxson, Andrew J Darwin
Mar 17, 2010·Infection and Immunity·Chelsea A SchianoWyndham W Lathem
Oct 4, 2011·Journal of Bacteriology·Sindhoora Singh, Andrew J Darwin
Nov 15, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marcelo S F PereiraDario S Zamboni
Mar 5, 2016·Molecular Microbiology·Jennifer R TannerAnn Karen C Brassinga

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