PMID: 3467360Jan 1, 1987Paper

Analysis of the mechanism, rate, and sites of proteolytic cleavage of human immunoglobulin D by high-pressure liquid chromatography

Proceedings of the National Academy of Sciences of the United States of America
N IshiokaF W Putnam

Abstract

The high susceptibility of human immunoglobulin D to proteolytic degradation affects its biological function, metabolism, and immunoassay. High-pressure liquid chromatography was used to investigate the mechanism and rate of limited proteolytic cleavage of IgD and also to identify, isolate, and quantify the reaction products. Within 1 to 5 min, tryptic digestion of native IgD almost quantitatively yields a labile Fab fragment, a stable Fc fragment, and a highly charged peptide derived from the hinge region. A galactosamine-rich glycopeptide from the hinge region increases inversely as the Fab is largely degraded to a series of peptides within 1 hr. In contrast, the Fc and the high-charge peptide resist proteolysis for more than 24 hr. The initial sites of cleavage of IgD occur in the hinge region at exposed secondary structures predicted to be beta-turns. Concomitant with removal of the galactosamine-rich glycopeptide at its carboxyl terminus, the Fd fragment is rapidly and rather randomly degraded, but the light chain is somewhat more resistant than the Fd section of the delta heavy chain. This study of the rapid rate of proteolysis of IgD explains the rarity with which intact IgD is found in human sera. It also raises questio...Continue Reading

References

Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·L C Lin, F W Putnam
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·N IshiokaF W Putnam
May 10, 1985·Science·M B WhiteF R Blattner
Feb 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S G DevareS A Aaronson
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·Y TakahashiF W Putnam
Feb 2, 1984·Nature·F R Blattner, P W Tucker
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·N TakahashiF W Putnam
Jan 1, 1982·Annals of the New York Academy of Sciences·F W PutnamB Debuire
Oct 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·F W PutnamL C Lin

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Citations

Apr 3, 2001·Molecular Immunology·J L Preud'hommeE Lelièvre
Feb 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Geir Å LøsetInger Sandlie
Aug 8, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·A ForsgrenK Riesbeck
Jun 29, 1988·Journal of Chromatography·F W Putnam, N Takahashi
Jul 1, 1990·Molecular and Cellular Biology·M B WhiteP W Tucker
Jun 16, 1989·Science·C S Gasser, R T Fraley

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