Analysis of the mechanisms of action of the Saccharomyces cerevisiae dominant lethal cdc42G12V and dominant negative cdc42D118A mutations.

The Journal of Biological Chemistry
C R DavisD I Johnson

Abstract

The Saccharomyces cerevisiae Cdc42p GTPase is localized to the plasma membrane and involved in signal transduction mechanisms controlling cell polarity. The mechanisms of action of the dominant negative cdc42(D118A) mutant and the lethal, gain of function cdc42(G12V) mutant were examined. Cdc42(D118A,C188S)p and its guanine-nucleotide exchange factor Cdc24p displayed a temperature-dependent interaction in the two-hybrid system, which correlated with the temperature dependence of the cdc42(D118A) phenotype and supported a Cdc24p sequestration model for the mechanism of cdc42(D118A) action. Five cdc42 mutations were isolated that led to decreased interactions with Cdc24p. The isolation of one mutation (V44A) correlated with the observations that the T35A effector domain mutation could interfere with Cdc42(D118A, C188S)p-Cdc24p interactions and could suppress the cdc42(D118A) mutation, suggesting that Cdc24p may interact with Cdc42p through its effector domain. The cdc42(G12V) mutant phenotypes were suppressed by the intragenic T35A and K183-187Q mutations and in skm1Delta and cla4Delta cells but not ste20Delta cells, suggesting that the mechanism of cdc42(G12V) action is through the Skm1p and Cla4p protein kinases at the plasma m...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Jan 2, 1992·Gene·T W ChristiansonP Hieter
Nov 11, 1991·Nucleic Acids Research·Y H ZhouR H Ebright
Oct 1, 1991·Current Opinion in Genetics & Development·J Chant, J R Pringle
Jun 28, 1991·Cell·D G Drubin
Nov 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·C T ChienS Fields
May 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·A A FinegoldF Tamanoi
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A Bender, J R Pringle
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·L A RokeachS O Hoch
Feb 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P MadauleA M Myers
Aug 1, 1984·Molecular and Cellular Biology·M Johnston, R W Davis
Sep 27, 1994·Proceedings of the National Academy of Sciences of the United States of America·H QadotaY Ohya
Sep 1, 1994·Trends in Genetics : TIG·J Chant
Apr 25, 1994·Nucleic Acids Research·H E FeilotterD Beach
Jul 1, 1993·Trends in Biochemical Sciences·M S Marshall
Dec 16, 1993·Nature·M S Boguski, F McCormick
Feb 1, 1994·Molecular and Cellular Biology·P J Miller, D I Johnson
Feb 9, 1996·Cell·D G Drubin, W J Nelson
Jun 14, 1996·The Journal of Biological Chemistry·P Mazur, W Baginsky
Apr 1, 1996·Current Opinion in Cell Biology·R A Cerione, Y Zheng
Jan 1, 1995·Cold Spring Harbor Symposia on Quantitative Biology·I HerskowitzM Peter
Jan 1, 1995·Cold Spring Harbor Symposia on Quantitative Biology·J R PringleH Fares

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Citations

Mar 31, 2004·Experimental Cell Research·Madhavi AgarwalDenis A Larochelle
Dec 17, 2009·The Journal of Biological Chemistry·Lynden JonesGary Eitzen
Jan 19, 2000·Molecular Biology of the Cell·K G KozminskiD G Drubin
Nov 29, 2002·Eukaryotic Cell·Sophia C UshinskyEkkehard Leberer
Nov 10, 2005·Eukaryotic Cell·Carolyn G Rasmussen, N Louise Glass
Feb 22, 2012·Biochemistry. Biokhimii︠a︡·M V Shepelev, I V Korobko
Jul 9, 2014·The Journal of Cell Biology·Pil Jung KangHay-Oak Park
Jan 5, 2006·Molecular Microbiology·Michael MahlertMichael Bölker
Sep 25, 1998·The Journal of Biological Chemistry·B Zhang, Y Zheng
Aug 23, 2019·Cell Reports·Patrícia M SilvaRobert A Arkowitz
Aug 28, 2004·Journal of Cell Science·Clemens HofmannJonathan Chernoff
Mar 6, 1999·Microbiology and Molecular Biology Reviews : MMBR·D I Johnson
Mar 5, 2003·Journal of Cell Science·Kylie J BoyceAlex Andrianopoulos

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