Analysis of the metabolome of Anopheles gambiae mosquito after exposure to Mycobacterium ulcerans

Scientific Reports
J Charles HoxmeierK M Dobos

Abstract

Infection with Mycobacterium ulcerans causes Buruli Ulcer, a neglected tropical disease. Mosquito vectors are suspected to participate in the transmission and environmental maintenance of the bacterium. However, mechanisms and consequences of mosquito contamination by M. ulcerans are not well understood. We evaluated the metabolome of the Anopheles gambiae mosquito to profile the metabolic changes associated with bacterial colonization. Contamination of mosquitoes with live M. ulcerans bacilli results in disruptions to lipid metabolic pathways of the mosquito, specifically the utilization of glycerolipid molecules, an affect that was not observed in mosquitoes exposed to dead M. ulcerans. These results are consistent with aberrations of lipid metabolism described in other mycobacterial infections, implying global host-pathogen interactions shared across diverse saprophytic and pathogenic mycobacterial species. This study implicates features of the bacterium, such as the putative M. ulcerans encoded phospholipase enzyme, which promote virulence, survival, and active adaptation in concert with mosquito development, and provides significant groundwork for enhanced studies of the vector-pathogen interactions using metabolomics prof...Continue Reading

References

Mar 1, 1972·Canadian Journal of Genetics and Cytology. Journal Canadien De Génétique Et De Cytologie·H Nickla
Oct 13, 2001·Infection and Immunity·K M DobosC H King
Jul 9, 2002·Molecular Microbiology·Catherine RaynaudMary Jackson
Dec 8, 2005·Annual Review of Entomology·David Stanley
Aug 5, 2006·Proteomics·Katherine HollywoodRoyston Goodacre
Jan 26, 2008·Emerging Infectious Diseases·Paul D R JohnsonJanet A M Fyfe
Mar 13, 2008·Proceedings of the National Academy of Sciences of the United States of America·Amit K Pandey, Christopher M Sassetti
Jul 19, 2008·The Journal of Clinical Investigation·Daniel CruzRobert L Modlin
Dec 2, 2008·BMC Bioinformatics·Ralf TautenhahnSteffen Neumann
Mar 26, 2010·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Paola D'Arrigo, Stefano Servi
Jul 3, 2010·EMBO Molecular Medicine·Mi-Jeong KimDavid G Russell
Aug 3, 2010·Applied and Environmental Microbiology·John R WallacePamela L C Small
Aug 14, 2010·PLoS Neglected Tropical Diseases·Janet A M FyfePaul D R Johnson
Nov 13, 2010·PLoS Neglected Tropical Diseases·Sacha J PidotTimothy P Stinear
Feb 18, 2011·Insect Biochemistry and Molecular Biology·Bradley J StevensonMark J I Paine
Jun 8, 2011·Microbiology and Molecular Biology Reviews : MMBR·Charles A Abbas, Andriy A Sibirny
Sep 29, 2011·PLoS Neglected Tropical Diseases·Caroline J LavenderPaul D R Johnson
Feb 7, 2012·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Katharina RöltgenGerd Pluschke
Mar 26, 2013·Journal of Visualized Experiments : JoVE·Corey D BroecklingJessica E Prenni
Jun 28, 2013·Malaria Journal·Sreelakshmi K SreenivasamurthyT S Keshava Prasad
Aug 7, 2013·Journal of Pharmaceutical and Biomedical Analysis·Helen G GikaIan D Wilson
Sep 1, 2007·Metabolomics : Official Journal of the Metabolomic Society·Lloyd W SumnerMark R Viant
Jan 15, 2014·Médecine et santé tropicales·L S Bayonne ManouB C de Jong
Jan 28, 2014·Biochimica Et Biophysica Acta·Stefano PiottoSimona Concilio
Feb 6, 2014·PLoS Neglected Tropical Diseases·Aaron MorrisJean-François Guégan
Apr 12, 2014·PLoS Neglected Tropical Diseases·Heather R WilliamsonPamela L C Small

❮ Previous
Next ❯

Citations

Oct 8, 2016·Scientific Reports·Apostolos KapranasDavid A Barrett
Mar 14, 2017·Scientific Reports·J Charles HoxmeierLars Eisen
Dec 5, 2015·Scientific Reports·Fatoumata NiangCaroline Demangel
Sep 22, 2017·The Canadian Journal of Infectious Diseases & Medical Microbiology = Journal Canadien Des Maladies Infectieuses Et De La Microbiologie Médicale·Rousseau DjouakaAnthony Ablordey
Feb 9, 2021·Cryobiology·Emily N GallichotteJennifer P Barfield

❮ Previous
Next ❯

Methods Mentioned

BETA
nuclear magnetic resonance

Software Mentioned

RAMClust
R
RamclustR
XCMS
ImageJ

Related Concepts

Related Feeds

Buruli Ulcer

Buruli ulcer is a progressive disease of subcutaneous tissues caused by mycobacterium ulcerans. Here is the latest research.

Related Papers

Médecine tropicale : revue du Corps de santé colonial
Portaels Françoise
Transactions of the Royal Society of Tropical Medicine and Hygiene
H S ThangarajMark Wansbrough-Jones
© 2021 Meta ULC. All rights reserved