Analysis of the Microbiota of Black Stain in the Primary Dentition

PloS One
Yue LiFeng Chen

Abstract

Black tooth stain is a characteristic extrinsic discoloration commonly seen on the cervical enamel following the contour of the gingiva. To investigate the relationship between black tooth stain and the oral microbiota, we used 16S rRNA gene sequencing to compare the microbial composition of dental plaque and saliva among caries-free children with and without black stain. Dental plaque and saliva, as well as black stain, were sampled from 10 children with and 15 children without black stain. Data were analyzed using the pipeline tool MOTHUR. Student's t-test was used to compare alpha diversities and the Mann-Whitney U test to compare the relative abundances of the microbial taxa. A total of 10 phyla, 19 classes, 32 orders, 61 families and 102 genera were detected in these samples. Shannon and Simpson diversity were found to be significantly lower in saliva samples of children with black stain. Microbial diversity was reduced in the black stain compared to the plaque samples. Actinomyces, Cardiobacterium, Haemophilus, Corynebacterium, Tannerella and Treponema were more abundant and Campylobacter less abundant in plaque samples of children with black stain. Principal component analysis demonstrated clustering among the dental pla...Continue Reading

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Citations

Jul 7, 2018·BDJ Open·M A Albelda-BernardoC C Sheth
Feb 9, 2020·European Archives of Paediatric Dentistry : Official Journal of the European Academy of Paediatric Dentistry·E GobbiA Majorana
Dec 20, 2019·Indian Journal of Dental Research : Official Publication of Indian Society for Dental Research·Ratika LihalaM Rajini
Oct 1, 2020·Scientific Reports·Verónica VesesChirag C Sheth
Mar 28, 2019·Frontiers in Cellular and Infection Microbiology·Yiping WeiXiaolei Wu
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Aug 9, 2020·Clinical and Experimental Dental Research·Nobuko NagaiSeikou Shintani
Aug 27, 2019·Journal of Esthetic and Restorative Dentistry : Official Publication of the American Academy of Esthetic Dentistry ... [et Al.]·Jorge Rodríguez-MartínezMaría-Jesús Sánchez-Martín
Jun 3, 2021·Journal of Paediatrics and Child Health·Katrina Zaballa, Anthony Liu
Aug 8, 2019·Caries Research·Vanessa Silva de RezendeRaquel Gonçalves Vieira-Andrade

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

MOTHUR
ACE

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