Analysis of the microRNA expression profile of normal human dermal papilla cells treated with 5α-dihydrotestosterone

Molecular Medicine Reports
Myung Joo LeeSungkwan An

Abstract

Clinical evidence has demonstrated that the accumulation of 5α-dihydrotestosterone (DHT) in dermal papilla cells (DPCs) is implicated in androgenetic alopecia. Whether this accumulation in DHT may have direct cellular effects leading to androgenetic alopecia remains to be elucidated. The present study aimed to determine whether DHT affects cell growth, cell cycle arrest, cell death, senescence and the induction of reactive oxygen species (ROS), and whether these effects are mediated by microRNA (miRNA)-dependent mechanisms. The cell viability and cell cycle were determined, levels of ROS were examined and senescence-associated β-galactosidase assays were performed in normal human DPCs (nHDPCs). Furthermore, miRNA expression profiling was performed using an miRNA microarray to determine whether changes in the expression levels of miRNA were associated with the cellular effects of DHT. The results revealed that DHT decreased cell growth by inducing cell death and G2 cell cycle arrest, and by increasing the production of ROS and senescence in the nHDPCs. In addition, 55 miRNAs were upregulated and 6 miRNAs were downregulated in the DHT-treated nHDPCs. Bioinformatic analysis demonstrated that the putative target genes of these upre...Continue Reading

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Citations

Oct 21, 2016·Reviews in Endocrine & Metabolic Disorders·Georgios NikolakisChristos C Zouboulis
Feb 15, 2018·International Journal of Molecular Sciences·Kwan Ho JeongHoon Kang
Feb 14, 2018·Journal of Cosmetic Dermatology·Edwin D Lephart
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Apr 16, 2019·Journal of Cellular Biochemistry·Youyu ShengQinping Yang
Jun 21, 2019·Scientific Reports·Bryan Siu-Yin HoPaul Lorenz Bigliardi
Oct 7, 2017·Molecular Medicine Reports·Dahye JooHwa Jun Cha

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