Analysis of the mismatch and insertion/deletion binding properties of Thermus thermophilus, HB8, MutS

Biochemical and Biophysical Research Communications
A WhitehouseD M Meredith

Abstract

The methyl-directed long patch repair pathway in Escherichia coli is involved in increasing the fidelity of replication specific repair of DNA polymerase incorporation errors. This pathway is mediated by three gene products, MutS, MutL, and MutH, which are conserved in higher eukaryotes. Mutations in human homologues of these proteins have been shown to be implicated in hereditary non-polyposis colorectal cancer (HNPCC). A MutS homologue has recently been identified in the extremely thermophilic bacterium, Thermus thermophilus. Here we describe analysis of the binding properties of this protein, which has indicated it can identify all specific base mismatches as well as one, two and three base pair insertion/deletion mutations. We therefore believe this protein may be generally useful for applications involving mismatch detection.

Citations

Nov 25, 2004·Nucleic Acids Research·Peter A CarrJoseph M Jacobson
Apr 1, 2005·Nucleic Acids Research·Brock F BinkowskiPeter J Belshaw
May 16, 2014·Nucleic Acids Research·Wen WanJiong Hong
Jun 1, 2016·Molecular Biotechnology·Michał Banasik, Paweł Sachadyn
Sep 16, 2017·Nucleic Acids Research·Nathan B LubockSriram Kosuri
Sep 6, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Kanako HoshiHiroshi Shimada
Feb 25, 2000·Mutation Research·S R RajskiJ K Barton
Mar 26, 2003·Protein Expression and Purification·Anna Stanisławska-SachadynJózef Kur

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