Analysis of the monovalent ion fluxes in U937 cells under the balanced ion distribution: recognition of ion transporters responsible for changes in cell ion and water balance during apoptosis

Cell Biology International
Alexey A VereninovA A Rubashkin

Abstract

Unidirectional (22)Na, Li(+) and Rb(+) fluxes and net fluxes of Na(+) and K(+) were measured in U937 human leukemic cells before and after induction of apoptosis by staurosporine (1 microM, 4 h) to answer the question which ion transporter(s) are responsible for changes in cell ion and water balance at apoptosis. The original version of the mathematical model of cell ion and water balance was used for analysis of the unidirectional ion fluxes under the balanced distribution of major monovalent ions across the cell membrane. The values of all major components of the Na(+) and K(+) efflux and influx, i.e. fluxes via the Na(+),K(+)-ATPase pump, Na(+) channels, K(+) channels, Na/Na exchanger and Na-Cl symport were determined. It is concluded that apoptotic cell shrinkage and changes in Na(+) and K(+) fluxes typical of apoptosis in U937 cells induced by staurosporine are caused by a complex decrease in the pump activity, Na-Cl symport and integral Na(+) channel permeability.

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Oct 16, 2012·Pflügers Archiv : European journal of physiology·J Marc SimardVolodymyr Gerzanich
Dec 12, 2012·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Valentina E YurinskayaAlexey A Vereninov
Oct 2, 2009·American Journal of Physiology. Cell Physiology·Georgina Valencia-CruzOxana R Dobrovinskaya
May 7, 2019·Frontiers in Cell and Developmental Biology·Valentina E YurinskayaAlexey A Vereninov
Jan 25, 2019·Scientific Reports·Irina MarakhovaNikolay Nikolsky
Nov 27, 2020·Frontiers in Cell and Developmental Biology·Valentina E YurinskayaAlexey A Vereninov
Nov 17, 2020·Frontiers in Cell and Developmental Biology·Priyanka S Rana, Michael A Model
Mar 4, 2019·Michael Model, Michael Model

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis