Analysis of the MUC1 gene and its polymorphism in Capra hircus

Journal of Dairy Science
P SacchiR Rasero

Abstract

The objective of our study was to demonstrate the existence of a repetitive region in the goat MUC1 gene and to develop a polymerase chain reaction (PCR) protocol to analyze its polymorphism in different breeds. Using 2 primers derived from the bovine MUC1 sequence, a PCR fragment was obtained and cloned. The sequence analysis shows that the repetitive region of goat MUC1 is an array of 60 bp repeats in accordance with the data reported for other species. The polypeptide sequences encoded by the consensus repeats of goat and bovine were exactly alike. A PCR protocol to improve the detection of goat MUC1 polymorphism was developed, and a total of 178 animals from 6 Italian breeds were analyzed. Fifteen different alleles, ranging in size from 1500 to 3000 bp, were found. The high number of alleles observed shows that the goat MUC1 is a hypervariable gene. These results are the basis for further investigations on the possible role of MUC1 polymorphism in the genetic control of disease susceptibility and production traits in the goat species.

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Citations

Nov 26, 2009·Animal Biotechnology·Yucai ZhengSuyu Jin
Jan 1, 2010·Genetics and Molecular Biology·Fabio Ricardo Pablos de SouzaLucia Regina Martelli
Feb 10, 2009·Veterinary Immunology and Immunopathology·Ezequiel LacunzaMaría V Croce
Feb 23, 2010·Journal of Dairy Science·C CeboP Martin
Jan 20, 2007·Journal of Dairy Science·R RaseroP Sacchi
Feb 14, 2012·Molecular Biology Reports·Chen XiaoWang Aiguo
Jun 3, 2011·Molecular Biology Reports·Fabio Ricardo Pablos de SouzaRoberto Rasero
Feb 17, 2007·Journal of Animal Breeding and Genetics = Zeitschrift Für Tierzüchtung Und Züchtungsbiologie·F R P de SouzaL R Martelli

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