Analysis of the neutral polysaccharide fraction of MCP and its inhibitory activity on galectin-3.

Glycoconjugate Journal
Xiaoge GaoYifa Zhou

Abstract

The pH-modified citrus pectin (MCP) has been demonstrated to inhibit galectin-3 in cancer progression. The components and structures of MCP related to this inhibition remained unknown. In this paper, we fractionated MCP on DEAE-cellulose column into a homogenous neutral fraction MCP-N (about 20 kDa) and a pectin mixture fraction MCP-A (wide molecular distribution on Sepharose CL-6B chromatography). Both MCP-N and MCP-A inhibited hemagglutination mediated by galectin-3 with minimum inhibition concentration (MIC) 625 and 0.5 μg/ml, respectively. MCP-N was identified to be a type I arabinogalactan (AG-I) with a main chain of β-1→4-galactan. MCP-N was digested by α-L-arabinofuranosidase to give its main chain structure fraction (M-galactan, around 18 kDa), which was more active than the original molecule, MIC 50 μg/ml. The acidic degradation of M-galactan increased the inhibitory activity, MIC about 5 times lower than M-galactan. These results above showed that the functional motif of the β-1→4-galactan fragment might lie in the terminal residues rather than in the internal region of the chain. Therefore, MCP-N and its degraded products might be developed to new potential galectin-3 inhibitors. This is the first report concerning t...Continue Reading

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Citations

Dec 22, 2015·Carbohydrate Polymers·Lionel LeclereCarine Michiels
Oct 3, 2015·Carbohydrate Polymers·Agnieszka WikieraBożena Stodolak
Apr 30, 2016·The Journal of Biological Chemistry·John StegmayrHakon Leffler
Nov 7, 2019·Nutrients·Isaac Eliaz, Avraham Raz
Apr 21, 2020·Canadian Journal of Physiology and Pharmacology·Jingang SunLianghua Chen
Sep 2, 2019·Carbohydrate Polymers·Sarah da Costa AmaralJoana Léa Meira Silveira
Jan 15, 2021·International Journal of Biological Macromolecules·Junwen ChengLiang He
May 1, 2021·International Journal of Molecular Sciences·Lukas PfeiferBirgit Classen
Jun 21, 2020·Fitoterapia·Oksana ZaitsevaTatyana Polezhaeva
Mar 3, 2019·Carbohydrate Polymers·Samira Bernardino Ramos do PradoJoão Paulo Fabi

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