PMID: 9536387Apr 16, 1998Paper

Analysis of the NF-kappa B p65 subunit, Fas antigen, Fas ligand and Bcl-2-related proteins in the synovium of RA and polyarticular JRA

Clinical and Experimental Rheumatology
M SioudO Førre

Abstract

To investigate the nuclear localization of the transcription factor NF-kappa B, the status of apoptosis and the expression of the Fas antigen, Fas ligand, Bcl-2, Bcl-xL and Bax by synovial cells. Electrophoresis mobility shift assay (EMSA), immunohistochemical staining, two colour-flow cytometry and the terminal deoxynucleotidyl transferase (TDT)-mediated dUTP nick end labelling (TUNEL)-technique were used. The NF-kappa B p65 subunit appears to be present in the nuclei of synovial tissue and fluid cells as detected by EMSA and immunohistochemical staining. Fas antigen and Fas ligand (L) are expressed on up to 90% and 11% of CD3+ synovial fluid (SF) cells, respectively. Both the Fas antigen and its ligand are also expressed by synovial tissue cells. Interestingly, freshly isolated SF T cells upregulated the expression of Bcl-xL as compared to Bcl-2. An overexpression of Bax compared to Bcl-xL was seen in the synovial tissues (ST) of patients with ongoing apoptosis, but not in patients with few apoptotic cells. NF-kappa B appear to be activated in vivo, both Fas and its ligand being expressed by synovial cells. Apoptosis is ongoing in the ST with significant patient variations. Such variations could be in part due to the level of...Continue Reading

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.