Analysis of the nuclear proteome of the resurrection plant Xerophyta viscosa in response to dehydration stress using iTRAQ with 2DLC and tandem mass spectrometry

Journal of Proteomics
Kamal Omer Abdalla, Mohamed Suhail Rafudeen

Abstract

Xerophyta viscosa Baker (family Velloziaceae) is a desiccation tolerant plant which survives extremes of dehydration down to 5% relative water content (RWC) and resumes full physiological activity within 80h of rehydration. The nuclear proteome of Xerophyta viscosa and its response to dehydration at 35% RWC as compared to fully hydrated plants was analysed using iTRAQ together with 2DLC and ESI-MS/MS. RWC at 35% is unique for desiccation tolerant species as it represents a distinct phase of the dehydration process where induction of late protection mechanisms are initiated. We reproducibly identified 122 proteins with confidence≥95% (ρ<0.05). In response to dehydration, 65% of the identified proteins had the same protein abundance as the hydrated, 22% were shown to be more abundant while 9.8% were less abundant. Classification of the nuclear proteins according to GO annotation showed that most proteins were part of cellular processes (77.43%) and had binding activity (85.47%) respectively. Ontological classification according to Interpro and Pfam databases categorized most nuclear proteins as part of gene regulation (21%) while the functions of the mapped proteins using MapMan showed involvement in protein synthesis (22%), degr...Continue Reading

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Citations

Jan 26, 2016·Journal of Proteomics·Xin Wang, Setsuko Komatsu
Jan 8, 2013·Trends in Plant Science·Olga ŠamajováJozef Šamaj
May 6, 2016·Frontiers in Plant Science·Deepti B GuptaNiranjan Chakraborty
Mar 5, 2013·International Journal of Molecular Sciences·Raquel FolgadoJean-Francois Hausman

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