PMID: 7543198Jul 11, 1995Paper

Analysis of the nucleic acid annealing activities of nucleocapsid protein from HIV-1

Nucleic Acids Research
M Lapadat-TapolskyJ L Darlix

Abstract

Retroviral nucleocapsid (NC) protein is an integral part of the virion nucleocapsid where it is in tight association with genomic RNA and the tRNA primer. NC protein is necessary for the dimerization and encapsidation of genomic RNA, the annealing of the tRNA primer to the primer binding site (PBS) and the initial strand transfer event. Due to the general nature of NC protein-promoted annealing, its use to improve nucleic acid interactions in various reactions can be envisioned. Parameters affecting NC-promoted nucleic acid annealing of NCp7 from HIV-1 have been analyzed. The promotion of RNA:RNA and RNA:DNA annealing by NCp7 is more sensitive to the concentration of MgCl2 than the promotion of DNA:DNA hybridization. Stimulation of complex formation for all three complexes was efficient at 0-90 mM NaCl, between 23 and 55 degrees C and at pH values between 6.5 and 9.5, inclusive. Parameters affecting NCp7-promoted hybridization of tRNA(Lys,3) to the PBS, which appears to be specific for NC protein, will be discussed. Results implicate the basic regions of NCp7, but not the zinc fingers, in promoting the annealing of complementary nucleic acid sequences. Finally, NCp7 strand transfer activity aids the formation of the most stable...Continue Reading

References

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Citations

Mar 30, 1999·Biochimica Et Biophysica Acta·B I KlasensB Berkhout
Mar 17, 1997·The EMBO Journal·D HarrichR B Gaynor
Sep 23, 2003·Nucleic Acids Research·Sébastien LyonnaisGilles Mirambeau
Sep 5, 2003·Nucleic Acids Research·G KrishnamoorthyYves Mély
Nov 7, 2003·Nucleic Acids Research·Nikolai WindbichlerRenée Schroeder
Aug 4, 2005·Nucleic Acids Research·Besik I KankiaKarin Musier-Forsyth
May 15, 1996·European Journal of Biochemistry·B Bonnet-MathonièreJ Paoletti
Jan 3, 2001·Molecular and Cellular Biology·S L Martin, F D Bushman
Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·B Chan, K Musier-Forsyth
Jan 20, 1999·Proceedings of the National Academy of Sciences of the United States of America·B ChanK Musier-Forsyth

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