Analysis of the OspE determinants involved in binding of factor H and OspE-targeting antibodies elicited during Borrelia burgdorferi infection in mice.

Infection and Immunity
Michael S MettsRichard T Marconi

Abstract

Immune evasion by Lyme spirochetes is a multifactorial process involving numerous mechanisms. The OspE protein family undergoes antigenic variation during infection and binds factor H (fH) and possibly FHL-1/reconectin. In Borrelia burgdorferi B31MI, the OspE family consists of three paralogs: BBL39 (ErpA), BBP38, and BBN38 (ErpP). BBL39 and BBP38 are identical and therefore are referred to here as BBL39. The goals of this study were to assess the specificity of the antibody (Ab) response to the OspE paralogs and to identify the domains or determinants of OspE that are required for the binding of fH and OspE-targeting Abs that develop during infection. Here we demonstrate that at least some of the anti-OspE Abs produced during infection are paralog specific and that Ab binding requires conformational determinants whose formation requires both the N- and C-terminal domains of OspE. The binding of fH to OspE was also found to be dependent on conformational determinants. It is also demonstrated here that all of the OspE paralogs expressed by B. burgdorferi B31MI are capable of binding fH. The binding of fH to members of the OspF protein family was also assessed. In contrast to an earlier report, no binding of BBO39 or BBR42 to hum...Continue Reading

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Citations

Jul 24, 2013·Trends in Microbiology·Jenifer CoburnGeorge Chaconas
Jan 11, 2012·Nature Reviews. Microbiology·Justin D RadolfLinden T Hu
Jun 4, 2011·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Daniel P MillerRichard T Marconi
Nov 6, 2007·Applied and Environmental Microbiology·Klára HanincováKlaus Kurtenbach
Aug 26, 2009·Infection and Immunity·Ashutosh VermaBrian Stevenson
Apr 20, 2006·Infection and Immunity·Ashutosh VermaBrian Stevenson
Jan 24, 2007·Journal of Bacteriology·Melisa S MedranoLinden T Hu
Apr 20, 2004·Journal of Bacteriology·Kelly BabbBrian Stevenson
Sep 15, 2012·Annual Review of Genetics·Dustin BrissonD Scott Samuels

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