PMID: 6730306Apr 1, 1984

Analysis of the outer membrane proteins of Bacteroides nodosus, the causal organism of ovine footrot

Veterinary Microbiology
D L EmeryD R Hewish

Abstract

Examination by SDS-PAGE of lithium acetate extracts of several strains of depiliated Bacteroides nodosus revealed 6 major outer membrane proteins (including pilin). The 5 membrane proteins exhibited approximate molecular weights of 75000, 50000, 38000, 34500 and 26500 whereas pilin had a MW of 17500 for the majority of strains. All proteins were accessible to lactoperoxidase-catalysed iodination and proteins 1, 2 and 5 were shown to be glycoproteins. Several attempts to isolate individual OMC proteins in pure form by selective solubilization and gel filtration were unsuccessful, but electroelution of individual outer membrane complex proteins resolved by SDS-PAGE provided sufficient quantities of antigen for immunization of sheep and for immunochemical analysis.

References

Nov 25, 1978·Lancet·D C CandyW C Marshall
Apr 1, 1983·Australian Veterinary Journal·K J FaheyB L Clark
Jan 1, 1982·Analytical Biochemistry·C M Tsai, C E Frasch

Citations

May 1, 1987·Veterinary Immunology and Immunopathology·D L Emery
Jan 1, 1991·Veterinary Microbiology·L J DepiazziW J Penhale
Mar 1, 1997·Veterinary Microbiology·R J WhittingtonE K Moses
Jan 12, 1993·Journal of Animal Breeding and Genetics = Zeitschrift Für Tierzüchtung Und Züchtungsbiologie·H W RaadsmaS C Brown
May 1, 1990·Australian Veterinary Journal·R J Whittington, D J Marshall
Apr 17, 2014·Vaccine·Om DhungyelR J Whittington
Oct 12, 2010·Veterinary Microbiology·G N Bennett, J G H Hickford
Apr 1, 1986·Australian Veterinary Journal·D J StewartI J O'Donnell
Feb 1, 1986·Australian Veterinary Journal·B G HandleyC L Schwartzkoff
Jan 1, 1987·Journal of Endodontics·P N Ramachandran Nair
Jul 1, 1996·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·R J Whittington
May 1, 1994·FEMS Microbiology Letters·L E SandercockR A Warren
Mar 1, 1990·Australian Veterinary Journal·R J WhittingtonM J Turner

Related Concepts

Antigens, Bacterial
Autoradiography
Outer Membrane Lipoproteins, Bacterial
Bacterial Proteins
Bacteroides
SDS-PAGE
Foot Rot
Immune Sera
Variolation
Cell Surface Proteins

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