Analysis of the paramagnetic copper(II) site of amicyanin by 1H NMR spectroscopy

Biochemistry
A P KalverdaG W Canters

Abstract

Application of the tailored pulse sequences like super-WEFT allows the direct observation of the hyperfine-shifted signals of the paramagnetic Cu(II) forms of blue copper proteins in solution. The signals can be assigned by applying 2D NMR techniques, like EXSY, to solutions containing a mixture of reduced and oxidized species. The Fermi contact shift is separated from the pseudocontact shift on the basis of the known g-tensor anisotropy of the Cu(II) state, allowing the determination of a number of hyperfine-splitting constants between protons on the Cu ligands and the unpaired electron. These results are used to quantify the spin density distribution over the Cu ligands. In amicyanin about 50%-60% of the unpaired electron density is found on the ligands. It appears possible to quantify the Cu-S(Met) interaction on the basis of the NMR results. Application of the technique to the wild type forms of amicyanin and azurin and to two active site mutants of amicyanin (His96Asp and a plastocyanin-amicyanin loop exchange mutant) shows that the Cu-S(Met) interaction parallels the rhombicity and axial distortion of the Cu site.

References

Dec 20, 1991·Journal of Molecular Biology·B A FieldsH C Freeman
Feb 5, 1990·Journal of Molecular Biology·C A CollyerH C Freeman
Jul 22, 1994·Journal of Molecular Biology·A P KalverdaG W Canters
Aug 1, 1994·European Journal of Biochemistry·B G Malmström

❮ Previous
Next ❯

Citations

Sep 19, 2003·Journal of Inorganic Biochemistry·Gianantonio BattistuzziMarco Sola
Mar 5, 2008·Journal of the American Chemical Society·Yong Zhang, Eric Oldfield
Aug 12, 2010·Journal of the American Chemical Society·María-Eugenia ZaballaAlejandro J Vila
Feb 13, 2003·European Journal of Biochemistry·Ioannis GelisLuisa Poggi
May 3, 2006·Proceedings of the National Academy of Sciences of the United States of America·Chan LiChristopher Dennison
Oct 20, 2005·Dalton Transactions : an International Journal of Inorganic Chemistry·Christopher Dennison
Mar 30, 2004·Proteins·Mark D Harrison, Christopher Dennison
Jun 23, 2007·Chembiochem : a European Journal of Chemical Biology·Ivano BertiniRoberta Pierattelli
Aug 24, 2006·Chemistry : a European Journal·Armand W J W TepperGerard W Canters
Jun 24, 2006·Chemistry : a European Journal·Katsuko Sato, Christopher Dennison
Oct 7, 1996·FEBS Letters·C DennisonG W Canters
Feb 6, 2008·Natural Product Reports·Christopher Dennison
Oct 8, 1999·Biospectroscopy·U KolczakG W Canters
Jan 4, 2020·Chemical Communications : Chem Comm·Marcos N MorgadaAlejandro J Vila
Jan 1, 2018·Quarterly Reviews of Biophysics·Candan Ariöz, Pernilla Wittung-Stafshede
Jul 1, 2016·Chemical Science·Jerome R RobinsonEric J Schelter
Mar 30, 2017·Dalton Transactions : an International Journal of Inorganic Chemistry·Annaleizle FerrancoHeinz-Bernhard Kraatz
Oct 13, 2018·Chemical Science·Andrés Espinoza-CaraAlejandro J Vila
Mar 9, 2021·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Rubin DasguptaMarcellus Ubbink
Mar 23, 2017·Inorganic Chemistry·Leah M SeebaldMaksim Royzen
Dec 25, 2003·Journal of the American Chemical Society·Wolfgang BermelRoberta Pierattelli

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.