Analysis of the phenotype and phagocytic activity of monocytes/macrophages from cattle infected with the bovine leukaemia virus

Veterinary Immunology and Immunopathology
D WerlingW Langhans

Abstract

The bovine leukaemia virus (BLV) is a retrovirus that infects mainly B lymphocytes of cattle, but proviral DNA can also be isolated from monocytes/macrophages. This study investigated the effect of BLV infection on surface antigens on freshly isolated peripheral blood monocytes and cultured monocyte-derived macrophages, with and without lipopolysaccharide (LPS) stimulation. The effect of BLV infection on phagocytic activity of CD14+ monocytes was also assessed. The percentage of monocytes expressing the surface antigens CD11b, CD32 (FcgammaRII), MHC class II and the surface antigen recognised by mAb DH59B were increased in BLV-positive cattle. In contrast, expression intensity of all markers was low in samples from BLV-positive cattle. CD14+ monocytes from BLV-positive cattle showed less Fcgamma-receptor-mediated phagocytosis compared to monocytes from BLV-negative cattle. After 7 days in culture, there was evidence for shedding/downregulation of surface antigens on monocyte-derived macrophages, in particular on cells from BLV-positive cattle. LPS stimulation decreased the percentage of cells expressing the measured markers in monocyte-derived macrophages taken from BLV-negative cattle, but not in cultures derived from BLV-posi...Continue Reading

References

Feb 15, 1992·Veterinary Immunology and Immunopathology·B C TaylorJ P Picanso
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·N SagataY Ikawa
Feb 1, 1987·The Journal of Experimental Medicine·F GotchB Moss
Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R KettmannG Marbaix
Jun 1, 1994·Veterinary Immunology and Immunopathology·H AdlerT W Jungi
Aug 1, 1996·Veterinary Immunology and Immunopathology·P SoppC J Howard
Nov 1, 1996·Veterinary Immunology and Immunopathology·Z TraininH Ungar-Waron

❮ Previous
Next ❯

Citations

Mar 12, 2003·Veterinary Immunology and Immunopathology·Catherine MüllerDirk Werling
Nov 7, 2001·Scandinavian Journal of Immunology·G AltreutherD Werling
Apr 14, 2000·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·R DusinskýW Ponti
Jan 3, 2015·Veterinary Immunology and Immunopathology·Meredith C Frie, Paul M Coussens
Sep 25, 2016·Research in Veterinary Science·Xing ChenShujun Zhang
Mar 30, 2016·Infection and Immunity·Katharina SobottaChristian Menge
Jan 10, 2018·Frontiers in Immunology·Jamal Hussen, Hans-Joachim Schuberth
Jan 21, 2000·The Journal of General Virology·A DoménechE Gómez-Lucía
Apr 25, 2015·Innate Immunity·Christian MengeGeorg Baljer
Sep 11, 1999·Journal of Virology·D Pyeon, G A Splitter
Jul 6, 2021·Frontiers in Veterinary Science·Ewerton de Souza LimaFernando Nogueira de Souza
Apr 4, 2017·Research in Veterinary Science·M G BlagitzA M M P Della Libera

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.