PMID: 11333314May 3, 2001Paper

Analysis of the phosphorylation level in guard-cell plasma membrane H+-ATPase in response to fusicoccin

Plant & Cell Physiology
T Kinoshita, K Shimazaki

Abstract

A fungal phytotoxin fusicoccin (FC) causes irreversible opening of stomata by activation of the plasma membrane H+-ATPase in guard cells. However, the mechanism by which FC activates the H+-ATPase is not fully understood with respect to the event of phosphorylation. In this study, we provide quantitative evidence that FC-dependent activation of H+-ATPase requires the phosphorylation of the C-terminus, and that FC maintains the activated state by preventing the dephosphorylation. The plasma membrane H+-ATPase in guard cells was phosphorylated on serine and threonine residues in the C-termini of both VHA1 and VHA2 by FC, and the phosphorylation level paralleled the rates of H+-pumping and ATP hydrolysis. An endogenous 14-3-3 protein was co-precipitated with the H+-ATPase, and the amount of 14-3-3 protein was proportional to the phosphorylation level of H+-ATPASE: The recombinant 14-3-3 protein bound to the C-terminus only when it was phosphorylated, even in the presence of FC. The phosphorylated C-terminus was dephosphorylated by alkaline phosphatase, and the dephosphorylation was completely prevented when the C-terminus had been incubated with both FC and 14-3-3 protein. The results suggest that FC activates the H+-ATPase by acc...Continue Reading

References

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Citations

Jun 5, 2003·Archives of Biochemistry and Biophysics·Hugh G Nimmo
Apr 12, 2006·Nature Cell Biology·Laura Serna
Mar 7, 2006·Nature Cell Biology·Mimi HashimotoKoh Iba
May 17, 2013·Proceedings of the National Academy of Sciences of the United States of America·Ingrid J De Vries-van LeeuwenAlbertus H de Boer
Feb 3, 2009·Journal of Experimental Botany·Caroline SirichandraJeffrey Leung
Jul 26, 2005·Plant Physiology·Shin-ichiro InoueKen-ichiro Shimazaki
Apr 13, 2012·Plant Physiology·Masaki OkumuraToshinori Kinoshita
Jan 11, 2007·Annual Review of Plant Biology·Ken-ichiro ShimazakiToshinori Kinoshita
Oct 28, 2006·Annual Review of Plant Biology·John M Christie
Feb 10, 2016·Frontiers in Plant Science·Valérie Cotelle, Nathalie Leonhardt
Aug 16, 2008·The Plant Journal : for Cell and Molecular Biology·Wei ZhangSarah M Assmann
May 23, 2012·The Plant Journal : for Cell and Molecular Biology·Céline ZimmerliYves Poirier
Apr 1, 2008·Plant Signaling & Behavior·Laura Serna
Jan 16, 2015·Plant & Cell Physiology·Yuriko KimuraToshinori Kinoshita
Apr 7, 2016·Plant & Cell Physiology·Yosuke TodaToshinori Kinoshita
Jul 11, 2002·Science's STKE : Signal Transduction Knowledge Environment·Nina V Fedoroff
Mar 31, 2017·Scientific Reports·Maki HayashiToshinori Kinoshita
Apr 14, 2017·Plant & Cell Physiology·Shin-Ichiro InoueKen-Ichiro Shimazaki
Apr 11, 2018·Plant & Cell Physiology·Shigeo TohToshinori Kinoshita
Dec 25, 2019·Journal of Experimental Botany·Jack S A MatthewsTracy Lawson
Mar 11, 2020·Annual Review of Plant Biology·Tracy Lawson, Jack Matthews
Aug 12, 2020·The Plant Journal : for Cell and Molecular Biology·Shin-Ichiro InoueKen-Ichiro Shimazaki

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