Analysis of the physicochemical state of C-reactive protein in different preparations including 2 certified reference materials

Clinical Chemistry
Malgorzata RzychonHeinz Schimmel

Abstract

Standardization of clinical measurements of C-reactive protein (CRP) is based on the availability of reference materials. The serum protein reference material ERM-DA470 was used as a calibrant for various commercially available immunoassays but has now been exhausted. The recently released ERM-DA470k/IFCC was intended to fully replace ERM-DA470. However, the new material was not suited for the certification of CRP because of a bias introduced by the lyophilization process that caused loss of about 20% of CRP measurable by routine immunoassays, compared with the nonlyophilized material that was stored in a liquid frozen state. We investigated the physicochemical state of CRP in a set of 4 lyophilized and 2 nonlyophilized serum-based CRP-containing materials by semi-native gel electrophoresis, Western blotting, and gel filtration. We detected a monomeric form of CRP (mCRP) in lyophilized materials at a concentration significantly higher than seen in the materials not subjected to lyophilization. Different reconstitution protocols led to variations of the monomeric CRP fraction found in reconstituted, previously lyophilized material. Most of the 20% loss in measured CRP after lyophilization of the material can be accounted for by ...Continue Reading

References

Apr 1, 1996·Nature Structural Biology·A K ShriveT J Greenhough
Jan 30, 1999·The International Journal of Biochemistry & Cell Biology·J J KreslB E Anderson
Sep 1, 2000·International Journal of Pharmaceutics·W Wang
Jun 5, 2003·Acta Crystallographica. Section D, Biological Crystallography·Yi WuSen-fang Sui
Oct 11, 2003·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Susanne B SchwedlerChristoph Wanner
Oct 11, 2005·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Lorraine MarnellTerry W Du Clos
Oct 1, 2008·Journal of Internal Medicine·J P CasasM B Pepys
Sep 22, 2009·Analytical Chemistry·Eric L Kilpatrick, David M Bunk
Apr 9, 2010·Clinical Chemistry and Laboratory Medicine : CCLM·Ingrid ZegersHeinz Schimmel

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Citations

May 11, 2013·Analytical and Bioanalytical Chemistry·Claudia Swart
Aug 25, 2015·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Yintao SuYunkun Wu
Mar 27, 2018·Veterinary Clinical Pathology·Francesca CanaliasAnna Bassols
Dec 29, 2020·Clinica Chimica Acta; International Journal of Clinical Chemistry·W Greg MillerUNKNOWN IFCC Working Group on Commutability in Metrological Traceability

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