PMID: 7529332Feb 1, 1995Paper

Analysis of the posttranslational modifications of the influenza virus M2 protein

Journal of Virology
L J HolsingerR A Lamb

Abstract

The sites of posttranslational modifications of the influenza A virus M2 protein were examined, and the effect of these modifications on the M2 protein ion channel activity was analyzed. Cysteine residues 17 and 19 in the M2 protein ectodomain form disulfide bonds. The cytoplasmic tail is posttranslationally modified by palmitoylation, and mutagenic studies support the view that cysteine residue 50 is the site for fatty acylation. In addition, the cytoplasmic tail of the M2 protein was found to be posttranslationally modified by the addition of phosphate to specific serine residues. Site-directed mutagenesis of serine residues in the M2 protein cytoplasmic tail, combined with phosphoamino acid analysis, indicated that serine residue 64 is the predominant site for phosphorylation but that serine residues 82, 89, and 93 were also phosphorylated but to much lesser extents. Disulfide-bond formation, palmitoylation, and phosphorylation occurred on M2 protein expressed in mammalian cells infected with influenza virus, in mammalian cells in which the M2 protein was expressed from DNA expression vectors, and when the M2 protein was expressed in oocytes of Xenopus laevis. The membrane currents of oocytes of Xenopus laevis expressing wil...Continue Reading

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Citations

Jan 11, 2008·Journal of Virology·Lisa A LopezBrenda G Hogue
Aug 2, 2008·Proceedings of the National Academy of Sciences of the United States of America·Xianghong JingRobert A Lamb
Oct 21, 2010·Virologica Sinica·Xin Liang, Zhi-Yuan Li
Jan 30, 2013·Biochemical Society Transactions·Michael VeitLarisa V Kordyukova
Dec 6, 2002·The Journal of General Virology·Eric Ka-Wai Hui, Debi P Nayak
Apr 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Andrea JegerlehnerMartin F Bachmann
Jan 12, 2007·Molecular BioSystems·Lawrence H Pinto, Robert A Lamb
Jul 11, 2009·Proceedings of the National Academy of Sciences of the United States of America·Chunlong MaLawrence H Pinto
May 3, 2012·Biology of the Cell·Michael Veit
May 19, 2017·Journal of Virology·Carina F PereiraPaul Digard
Dec 25, 2019·The Biochemical Journal·Mohamed Rasheed GadallaMichael Veit
May 27, 2020·Annual Review of Virology·Anthony R DawsonAndrew Mehle
Jan 13, 2006·The Journal of Biological Chemistry·Lawrence H Pinto, Robert A Lamb
Apr 20, 2005·Proceedings of the National Academy of Sciences of the United States of America·Rose A Gubitosi-KlugRichard W Gross
Mar 18, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Hitesh K JindalGideon Koren
Feb 8, 2013·Protein Science : a Publication of the Protein Society·Huan-Xiang Zhou, Timothy A Cross
Jan 11, 2011·The Journal of Biological Chemistry·Michael J Shipston
Aug 17, 2002·The Journal of Biological Chemistry·Yajun TangLawrence H Pinto
Oct 24, 2003·Protein Science : a Publication of the Protein Society·Changlin TianTimothy A Cross
Mar 12, 2010·Journal of Virology·Jeremy S RossmanRobert A Lamb
Nov 18, 2010·Virus Genes·Seyyed Mahmoud Ebrahimi, Majid Tebianian
Dec 30, 2014·Protein Science : a Publication of the Protein Society·Shenstone HuangKathleen P Howard
May 14, 2014·The Journal of General Physiology·Michael J Shipston
Sep 12, 2013·Protein Science : a Publication of the Protein Society·Shu Yu LiaoMei Hong
Oct 26, 1999·Viral Immunology·S LudwigT Wolff
Oct 9, 2002·Journal of Virology·Ann H ReidJeffery K Taubenberger
Jun 9, 2006·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Lawrence H Pinto, Robert A Lamb
Feb 20, 1999·The Journal of Biological Chemistry·C S GandhiL H Pinto
Nov 13, 2012·PLoS Pathogens·Edward C HutchinsonErvin Fodor
Jun 29, 2004·European Biophysics Journal : EBJ·Cornelia SchroederTse-I Lin

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