Analysis of the potential of cancer cell lines to release tissue factor-containing microvesicles: correlation with tissue factor and PAR2 expression

Thrombosis Journal
Camille EttelaieA Maraveyas

Abstract

Despite the association of cancer-derived circulating tissue factor (TF)-containing microvesicles and hypercoagulable state, correlations with the incidence of thrombosis remain unclear. In this study the upregulation of TF release upon activation of various cancer cell lines, and the correlation with TF and PAR2 expression and/or activity was examined. Microvesicle release was induced by PAR2 activation in seventeen cell lines and released microvesicle density, microvesicle-associated TF activity, and phoshpatidylserine-mediated activity were measured. The time-course for TF release was monitored over 90 min in each cell line. In addition, TF mRNA expression, cellular TF protein and cell-surface TF activities were quantified. Moreover, the relative expression of PAR2 mRNA and cellular protein were analysed. Any correlations between the above parameters were examined by determining the Pearson's correlation coefficients. TF release as microvesicles peaked between 30-60 min post-activation in the majority of cell lines tested. The magnitude of the maximal TF release positively correlated with TF mRNA (c = 0.717; p < 0.001) and PAR2 mRNA (c = 0.770; p < 0.001) expressions while the percentage increase correlated with PAR2 mRNA (c...Continue Reading

References

May 11, 2000·Proceedings of the National Academy of Sciences of the United States of America·E CamererS R Coughlin
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Aug 13, 2002·The Biochemical Journal·Steven J ComptonMorley D Hollenberg
Mar 25, 2004·The Journal of Biological Chemistry·Jasimuddin Ahamed, Wolfram Ruf
Aug 12, 2005·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Einar S Breimo, Bjarne Østerud
Aug 27, 2005·Thrombosis Research·Rafail I KushakEric F Grabowski
Feb 10, 2006·Blood Cells, Molecules & Diseases·Robert Flaumenhaft
Jul 15, 2006·Journal of Thrombosis and Haemostasis : JTH·M L RandJ Freedman
Apr 25, 2008·Journal of Thrombosis and Haemostasis : JTH·M DavilaJ L Francis
Sep 18, 2008·Journal of Thrombosis and Haemostasis : JTH·A A KhoranaM B Taubman
Jun 9, 2009·Journal of Thrombosis and Haemostasis : JTH·M E T TesselaarS Osanto
Nov 5, 2010·Seminars in Thrombosis and Hemostasis·Nigel S Key, Nigel Mackman
Jan 15, 2011·Platelets·A RankR Pihusch
Mar 5, 2011·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Aurélien DellucKarine Lacut
Sep 13, 2011·Thrombosis Research·Paolo BucciarelliPier Mannuccio Mannucci
Mar 10, 2012·Thrombosis Research·Saulius Butenas, Jolanta Krudysz-Amblo
Mar 16, 2012·Thrombosis Research·James H MorrisseyChad M Rienstra
Jun 15, 2012·Thrombosis Research·Anna FalangaMarina Marchetti
Sep 26, 2012·Journal of Thrombosis and Haemostasis : JTH·L V M Rao, U R Pendurthi
Oct 3, 2012·Thrombosis Research·Wolfram Ruf
Oct 23, 2012·Thrombosis Research·Johannes ThalerJohannes A Hainfellner
Feb 13, 2013·European Journal of Clinical Investigation·Johannes ThalerIngrid Pabinger
Mar 26, 2013·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Bashir A LwaleedAlan J Cooper
Apr 26, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Lichao HuJinchuan Yan
Apr 30, 2013·Journal of Thrombosis and Haemostasis : JTH·H KothariL V M Rao
Aug 3, 2013·Blood·Ingrid PabingerCihan Ay
Aug 27, 2013·Translational Research : the Journal of Laboratory and Clinical Medicine·Johannes ThalerCihan Ay
Nov 22, 2013·Thrombosis and Haemostasis·Mary E W CollierCamille Ettelaie
Nov 29, 2013·Thrombosis and Haemostasis·Narjes Tavoosi, James H Morrissey

❮ Previous
Next ❯

Citations

Aug 7, 2018·Cell Biology International·Frank GieselerFanny Ender
Jul 2, 2019·Current Opinion in Hematology·Yohei Hisada, Nigel Mackman
Sep 3, 2020·Clinical and Applied Thrombosis/hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis·Marwa S Hamza, Shaker A Mousa
Oct 13, 2020·Frontiers in Oncology·Serena Lucotti, Ruth J Muschel
Jul 25, 2021·International Journal of Molecular Sciences·Tyrone L R HumphriesDavid A Vesey

❮ Previous
Next ❯

Methods Mentioned

BETA
glycosylation

Software Mentioned

Statistical Package for the Social Sciences ( SPSS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.