PMID: 2009966Mar 11, 1991Paper

Analysis of the protein products encoded by variant glucokinase transcripts via expression in bacteria

FEBS Letters
C QuaadeC B Newgard

Abstract

Five variant transcripts of the single rat glucokinase gene have been described that are naturally expressed in islets of Langerhans, liver and anterior pituitary. Four of these were prepared as cDNA and expressed in bacteria in order to begin to address their physiological roles. Expression of constructs pGKB1 (normal islet/pituitary glucokinase) and pGKL1 (normal liver glucokinase) resulted in a glucose-dependent, glucokinase-like activity, 7-fold and 45-fold, respectively, above background. Expression of pGKB3 (variant islet/pituitary glucokinase) and pGKL2 (variant liver glucokinase) in contrast, did not result in any glucokinase-like activity.

References

Jul 5, 1990·European Journal of Biochemistry·W J MalaisseE Van Schaftingen
Aug 15, 1990·The Biochemical Journal·D J Hayzer, P B Iynedjian
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M A MagnusonD K Granner
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·P B IynedjianC B Newgard
Dec 15, 1989·Biochemical and Biophysical Research Communications·C T ChienS J Pilkis
Jan 1, 1986·Diabetes/metabolism Reviews·M D Meglasson, F M Matschinsky
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·P B IynedjianA E Renold

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Citations

Feb 1, 1992·Journal of Cellular Biochemistry·M A Magnuson
Jan 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S D HughesC B Newgard

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