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Analysis of the radioimmunoassay for gonadotropin-releasing hormone (GnRH): studies on the effect of radioiodinated GnRH

The Journal of Clinical Endocrinology and Metabolism

Dec 1, 1975

Lynwood G ClemensW C Dermody

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Abstract

When GnRH is radioiodinated by the chloramine-T method, two immunoreactive labeled species are formed at pH 6.5 with a chloramine-T: GnRH molar ratio of 11:1, whereas four bands (I, IIa, IIb, and III) are separated by polyacrylamide gel electrophoresis when the hormone is iodinated at p...read more

Mentioned in this Paper

Plasma
Recombinant Gonadorelin
Gonadorelin Hydrochloride
GNRH1 gene
Immune Sera
Ethanol Measurement
Organum Vasculosum Laminae Terminalis
Pineal Gland
Tissue Extracts
Iodine Radioisotopes

Analysis of the radioimmunoassay for gonadotropin-releasing hormone (GnRH): studies on the effect of radioiodinated GnRH

The Journal of Clinical Endocrinology and Metabolism

Dec 1, 1975

Lynwood G ClemensW C Dermody

PMID: 1416

DOI: 10.1210/jcem-41-6-1058

Abstract

When GnRH is radioiodinated by the chloramine-T method, two immunoreactive labeled species are formed at pH 6.5 with a chloramine-T: GnRH molar ratio of 11:1, whereas four bands (I, IIa, IIb, and III) are separated by polyacrylamide gel electrophoresis when the hormone is iodinated at p...read more

Mentioned in this Paper

Plasma
Recombinant Gonadorelin
Gonadorelin Hydrochloride
GNRH1 gene
Immune Sera

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