Analysis of the Rb gene and cyclin-dependent kinase 4 inhibitor genes (p16INK4 and p15INK4B) in human ovarian carcinoma cell lines

Experimental Cell Research
Y YaginumaM Ishikawa

Abstract

In the present study, we analyzed human ovarian carcinoma cell lines for abnormalities in the tumor suppressor gene Rb (retinoblastoma) and in cyclin-dependent kinase 4 (CDK4) inhibitor genes (p16INK4 and p15INK4B) using molecular biology techniques. For the Rb gene, in all six cell lines (PA-1, Caov-3 and -4, OVCAR-3, SK-OV-3, and Kuramochi), Rb gene abnormality was not detected using Southern blotting. In the Caov-3 cell line transcripts were not detectable by either Northern blot or polymerase chain reaction. Sequence analysis of the entire coding region of the Rb gene revealed point mutations (AAC to GAC) resulting in codon 123 (Asn to Asp) changes in the Caov-4 cell line. In the PA-1 cell line both wild-type Rb and mutant-type Rb (codon 798: CGG to TGG) were expressed, and in the OVCAR-3 cell line both wild-type Rb and mutant-type Rb (codon 704: ATG to GTG) were expressed. In four of six human ovarian carcinoma cell lines Rb gene abnormality was detected. For the p16INK4 and p15INK4B genes, only the SK-OV-3 cell line had abnormalities. There was a gene rearrangement or minor deletion of the p16INK4 gene in the SK-OV-3 cell line, while the p15INK4B gene was deleted in this cell line. In the SK-OV-3 cell line no mRNAs of p16...Continue Reading

References

Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S Munro, T Maniatis
Feb 17, 1984·Science·L A FeigG M Cooper

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Citations

Jul 17, 1998·Journal of the National Cancer Institute·D W AbbottJ T Holt
Mar 9, 2011·The Journal of Clinical Investigation·Claire M ConnellIain A McNeish
Feb 2, 2012·The Journal of Clinical Investigation·Uma M Sachdeva, Joan M O'Brien
Mar 20, 2010·Cancer Cell International·Shauna A HenleyFrederick A Dick
Jul 6, 2010·Molecular Cancer·Magdalena B FlakIain A McNeish
Oct 20, 2004·The Journal of Gene Medicine·John T LamAkseli Hemminki
May 29, 2016·Gynecologic Oncology·Lidia HernandezChristina M Annunziata
Feb 5, 2000·Molecular Carcinogenesis·Y YaginumaM Ishikawa
Dec 19, 2007·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·Eiwa IshidaNoboru Konishi
Apr 19, 2013·Journal of Cancer Research and Clinical Oncology·Linjuan XuZehua Wang
Jun 7, 2019·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Omar KujanCamile S Farah
Jun 7, 2019·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Omar KujanCamile S Farah
Nov 27, 2014·G3 : Genes - Genomes - Genetics·Xiaofeng DaiZhonghu Bai
Aug 3, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Huiying YanHaishan Huang
Oct 19, 2006·Cancer Research·Honglin SongPaul D P Pharoah
Dec 30, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Giuseppina D'AndrilliAntonio Giordano

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