PMID: 8955415Dec 1, 1996Paper

Analysis of the region between amino acids 30 and 42 of intact UmuD by a monocysteine approach

Journal of Bacteriology
A GuzzoG C Walker

Abstract

On the basis of characterizations of a set of UmuD monocysteine derivatives, we had suggested that positions 24, 34, and 44 are closer to the intact UmuD homodimer interface than other positions tested (M. H. Lee, T. Ohta, and G. C. Walker, J. Bacteriol. 176:4825-4837, 1994). Because this region of UmuD also appeared to be important for interactions with RecA, we followed up on our previous study by constructing a second set of monocysteine UmuD derivatives with single cysteine substitutions at positions 30 to 42. We found that like the VC34 mutant, UmuD derivatives with monocysteine substitutions at positions 32 and 35 showed deficiencies in in vivo and in vitro RecA-mediated cleavage as well as in UV mutagenesis, suggesting that the position 32 to 35 region may be important for RecA-mediated cleavage of UmuD. Interestingly, UmuD with monocysteine substitutions at residues 33 and 40 showed a reduction in UV mutagenesis while retaining the ability to be cleaved by RecA in vivo, suggesting a deficiency in the subsequent role of the UmuD' derivatives in mutagenesis. All of the UmuD monocysteine derivatives in the position 30 to 42 series purified indistinguishably from the wild-type protein. The observations that purified protein...Continue Reading

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Citations

Feb 11, 2004·Journal of Biomolecular Structure & Dynamics·Rajagopal Chattopadhyaya, Atasi Pal
Jan 5, 2006·Molecular Microbiology·Penny J BeuningGraham C Walker
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·T OppermanG C Walker
Dec 2, 2010·The Journal of Biological Chemistry·Jaylene N OllivierrePenny J Beuning
Mar 3, 2010·Proceedings of the National Academy of Sciences of the United States of America·Yubin ZhouPatrick G Hogan
Apr 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Mark D SuttonGraham C Walker
Nov 23, 2000·Journal of Biomolecular Structure & Dynamics·R ChattopadhyayaV M Namboodiri
Jan 25, 2008·Proceedings of the National Academy of Sciences of the United States of America·S M SimonG C Walker
Feb 9, 2006·The Journal of Biological Chemistry·Penny J BeuningGraham C Walker
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·G C Walker
Aug 29, 1998·Journal of Bacteriology·M McLeniganR Woodgate
Jun 2, 2009·Journal of Bacteriology·Brenna W ShurtleffPenny J Beuning
Mar 13, 2019·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Mark M MuenterPenny J Beuning
May 9, 2001·Mutation Research·G C Walker
Nov 1, 2012·EcoSal Plus·Alexandra VaismanRoger Woodgate
Dec 1, 1996·Journal of Bacteriology·M H LeeG C Walker
Apr 30, 1998·Genetics·B T Smith, G C Walker

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