Analysis of the relationship between invasive capability of Helicobacter pylori and gastroduodenal diseases

Journal of Medical Microbiology
Xiaoyan ZhangFeifei She

Abstract

Helicobacter pylori (H. pylori) may enter into host cells, maybe as a facultative intracellular pathogen. This study aims to reveal the roles of internalized H. pylori in the bacterial pathopoiesis. Transmission electron microscopy was used to observe the invasion of H. pylori. Invasion rates of H. pylori (two standard strains and 43 clinical strains) were examined by gentamicin invasion assay. The cagA, cagE and vacA genes of H. pylori were detected by PCR. The cagA 3'region (cagA-EPIYA) of each strain was sequenced. The secretion of IL-8 from AGS cells and activity of NF-κB induced by intracellular H. pylori were tested by ELISA and the dual-luciferase reporter assay system, respectively. It was found that H. pylori could adhere to and invade AGS cells, then continue to survive and multiply in the cytoplasm. The average invasion rate of H. pylori gastric cancer plants and that of ulcer plants were both higher than that of gastritis plants (P ≈ 0.0001). In the clinical strains, cagA, vacA and cagE were all positive; cagA-EPIYA genotypes included ABD 90.7% (39/43) and ABBD 9.3% (4/43), all without comparability. Notably, the average invasion rate of H. pylori vacA s1c-i1-m1b plants was higher than that of vacA s1c-i1-m2 plants ...Continue Reading

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Citations

Nov 7, 2017·FEMS Microbiology Letters·Xiaoyan ZhangFeifei She
Dec 7, 2016·Frontiers in Cellular and Infection Microbiology·Ying HuangNong-Hua Lu
Jun 23, 2020·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Andrea BeerAthanasios Makristathis
Jul 1, 2018·Comprehensive Reviews in Food Science and Food Safety·Qing LiuHua-Bin Li

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