Analysis of the relationship between Scl transcription factor complex protein expression patterns and the effects of LiCl on ATRA-induced differentiation in blast cells from patients with acute myeloid leukemia

Leukemia Research
Anna M RiceA C Sartorelli

Abstract

Exogenous expression of the transcription factor Scl (Tal1) in WEHI-3B D+ myelomonocytic leukemia cells interferes with their capacity to respond to all-trans retinoic acid (ATRA) induced differentiation; combination of ATRA with LiCl, however, circumvents the inhibition of differentiation produced by Scl. To gain information on the possible involvement of this transcription factor in the non-responsiveness of acute myelocytic leukemia (AML) patients to ATRA, we compared the endogenous expression levels of Scl and its transcription complex partners [i.e., Rbtn1 (LMO1), Rbtn2 (LMO2), Ldb1, and GATA family proteins] in leukemic blast cells from patients with AML and acute promyelocytic leukemia (APL), and determined the effects of lithium chloride alone or in combination with ATRA on the capacity of blast cells to differentiate during short-term ex vivo culture. Levels of Scl, Rbtn2, GATA1, and Ldb1 expression were comparable in AML and APL blasts, while the levels of expression of Rbtn1, GATA2, and GATA3 were absent or markedly lower in APL cells. Differentiation markers (cell surface myeloid antigens CD11b, CD15, CD14, and CD33) were also analyzed in blast cells. ATRA produced changes in at least one surface antigen differentia...Continue Reading

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Citations

Jul 16, 2010·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Ying XuSuoqin Tang
Dec 6, 2012·Leukemia Research and Treatment·Geoffrey Brown, Philip Hughes
Jan 31, 2006·Expert Opinion on Therapeutic Targets·Øystein BruserudBjørn Tore Gjertsen
Oct 17, 2008·Expert Opinion on Investigational Drugs·Anita RyningenOystein Bruserud
Jan 5, 2006·Critical Reviews in Clinical Laboratory Sciences·K N LeungM C Fung
Mar 21, 2021·Cell Death and Differentiation·Magali HumbertMario P Tschan

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