PMID: 2506929Sep 21, 1989Paper

Analysis of the reversible binding of virginiamycin M to ribosome and particle functions after removal of the antibiotic

Biochimica Et Biophysica Acta
E NyssenC Cocito

Abstract

Type A synergimycins (VM) were shown to act catalytically and to induce two ribosomal alterations: (a) inability to promote polypeptide synthesis; (b) high-affinity binding of type B synergimycins (VS). A claim for irreversible binding of type A synergimycins to ribosomes has promoted the present reinvestigation. Submission of ribosomes from VM-treated bacteria to a purification procedure (supposed to remove the drug, according to a low association constant previously reported) yielded particles still holding residual VM. The formation of VM.ribosome complexes, more stable than previously inferred but without covalent linkage, was deduced from the extractability of complexed VM by organic solvents. Moreover, incubation of these complexes with increasing amounts of anti-VM immunoglobulins progressively restored ribosome activity in protein synthesis. Binding of VS to ribosomes, by fluorimetric titrations in the presence of substoichiometric concentrations of VM, was incompatible with catalytic action of type A synergimycins. Ribosomes from VM-treated bacteria displayed also a higher affinity for VS than did control ribosomes. This property did not disappear when ribosome.VM complexes were incubated with anti-VM IgG, nor when VM-...Continue Reading

References

Oct 25, 1978·Molecular & General Genetics : MGG·R ParfaitC Cocito
Oct 25, 1978·Molecular & General Genetics : MGG·C Cocito, M Di Giambattista
Apr 15, 1977·European Journal of Biochemistry·A Contreras, D Vázquez
Nov 10, 1988·Biochimica Et Biophysica Acta·G ChinaliC Cocito
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R Parfait, C Cocito
Jul 7, 2009·Archives of Physical Medicine and Rehabilitation·Michael I WeintraubSteven P Cole
Apr 4, 2013·Proceedings of the National Academy of Sciences of the United States of America·Edward L MilesPeter Sutovsky

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Citations

Feb 2, 2011·Proceedings of the National Academy of Sciences of the United States of America·Matthew J BelousoffAda Yonath
Jun 19, 2002·Antimicrobial Agents and Chemotherapy·Brigitte MalbrunyRoland Leclercq
Nov 26, 2009·Critical Reviews in Biochemistry and Molecular Biology·Daniel N Wilson
Jul 5, 2005·Expert Opinion on Investigational Drugs·D Beyer, K Pepper
Feb 15, 2001·Expert Opinion on Investigational Drugs·G Bonfiglio, P M Furneri
Dec 13, 2005·Biological Chemistry·Daniel N WilsonPaola Fucini

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