Analysis of the RNA of Potato yellow vein virus: evidence for a tripartite genome and conserved 3'-terminal structures among members of the genus Crinivirus

The Journal of General Virology
Ioannis C LivieratosR H A Coutts

Abstract

Double-stranded RNA preparations produced from potato plants graft-inoculated with a Peruvian isolate of Potato yellow vein virus (PYVV; genus Crinivirus, family Closteroviridae) contain five RNA species denoted RNA 1, RNA 2, RNA 3, x and y of approximately 8, 5.3, 3.8, 2.0 and 1.8 kbp, respectively. The complete nucleotide sequences of PYVV RNAs 1, 2 and 3 and Northern hybridization analysis showed that PYVV RNA 1 contained the replication module and an additional open reading frame (p7), while two distinct species, RNAs 2 and 3, contain the Closteroviridae hallmark gene array. Pairwise comparisons and phylogeny of genome-encoded proteins showed that PYVV shares significant homology with other criniviruses but is most closely related to the Trialeurodes vaporariorum-vectored Cucumber yellows virus. Secondary structure prediction of the 3'-untranslated regions of all three PYVV RNAs revealed four conserved stem-loop structures and a 3'-terminal pseudoknot structure, also predicted for all fully characterized members of the genus Crinivirus and some members of the genera Closterovirus and Ampelovirus.

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Citations

Jul 9, 2005·Archives of Virology·W M WintermantelI E Tzanetakis
Sep 13, 2005·Archives of Virology·E EliascoR H A Coutts
Dec 24, 2005·Archives of Virology·G LozanoJ Navas-Castillo
Jul 8, 2011·Archives of Virology·Ioannis E TzanetakisJing Zhou
May 17, 2011·Annual Review of Phytopathology·Jesús Navas-CastilloSonia Sánchez-Campos
Mar 15, 2006·Virus Research·Valerian V DoljaJari P T Valkonen
Sep 7, 2011·Virus Research·Jeremy R ThompsonKeith L Perry
Dec 15, 2010·Virus Genes·Michael J MelzerJohn S Hu
Nov 3, 2020·AIMS Microbiology·Sergey Y Morozov, Andrey G Solovyev
Jun 8, 2021·The Journal of General Virology·Ángela NiñoLiliana Franco-Lara
Aug 13, 2021·Frontiers in Microbiology·Elisa Navas-HermosillaJesús Navas-Castillo

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